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PMID: 18055554 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Interleukin-4 does not influence development of hypercholesterolemia or angiotensin II-induced atherosclerotic lesions in mice.

The American journal of pathology ·Vol. 171 ·No. 6 ·2007-12-00 ·Pages 2040-7

King VL, Cassis LA, Daugherty A

Abstract

Interleukin-4 (IL-4) has been detected in both human and mouse atherosclerotic lesions, although its effects on the development of the disease are undefined. We determined the role of IL-4 in the most commonly used murine models of atherosclerosis by defining the effects of exogenous delivery and genetic deficiency of this cytokine on both hypercholesterolemia and AngII-induced atherosclerosis in apolipoprotein E (apoE)(-/-) mice and different dietary stimuli in low-density lipoprotein (LDL) receptor(-/-) mice. Exogenous administration of IL-4 (1.1 ng g(-1) day(-1) i.p. for 30 days) into female apoE(-/-) mice had no effect on lesion size or composition in mice fed normal or saturated fat diets. Also, IL-4 deficiency had no significant effect on the size or composition of atherosclerotic lesions in two vascular areas of male and female apoE(-/-) mice fed either a normal or saturated fat diet. IL-4 deficiency was also studied in age-matched male mice infused with AngII (1000 ng kg(-1) min(-1)) for 28 days. Whereas AngII infusion augmented atherosclerotic lesion formation, IL-4 deficiency did not influence atherosclerotic lesion size or composition. Finally, different dietary stimuli also had no effect on atherosclerotic lesion size in female LDL receptor(-/-) mice. These data demonstrate that IL-4 does not significantly influence the development of atherosclerotic lesions in apoE(-/-) mice of either gender or in female LDL receptor(-/-) mice, irrespective of the mode of induction of atherosclerosis.

MeSH Terms
Angiotensin II/administration & dosage,toxicity Animals Apolipoproteins E/genetics Atherosclerosis/chemically induced,metabolism,pathology Disease Models, Animal Female Hypercholesterolemia/metabolism Interleukin-4/metabolism,pharmacology Male Mice Mice, Mutant Strains Receptors, LDL/genetics
Chemicals
Apolipoproteins E Receptors, LDL Angiotensin II Interleukin-4
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
King Victoria L
Cardiovascular Research Center, Wethington Building, Room 562, University of Kentucky, Lexington, KY 40536-0200, USA. vicky.king@uky.edu
Cassis Lisa A
Daugherty Alan
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.2353/ajpath.2007.060857
Email
vicky.king@uky.edu
Published
2007-12-00
Epub
2007-00-30
Pages
2040-7
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2111126
Subset
IM
Grants
NHLBI NIH HHS · R01 HL062846 · United States
NHLBI NIH HHS · HL62846 · United States
NHLBI NIH HHS · HL55487 · United States
External Links
PubMed source DOI: 10.2353/ajpath.2007.060857 PMC Full Text View journal details
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