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PMID: 18047641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Application of phage display to high throughput antibody generation and characterization.

Genome biology ·Vol. 8 ·No. 11 ·2007-00-00 ·Pages R254

Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SDj, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J

Abstract

We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation.

MeSH Terms
Animals Antibody Formation Antibody Specificity Bacteriophages/genetics Base Sequence DNA Primers Enzyme-Linked Immunosorbent Assay Flow Cytometry Gene Expression Profiling Humans Immunohistochemistry In Situ Hybridization Mice Recombinant Proteins/biosynthesis,genetics,immunology
Chemicals
DNA Primers Recombinant Proteins
Authors & Affiliations
29 authors, click to expand affiliations / ORCID
Schofield Darren J
Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK. Darren.Schofield@abcam.com
Pope Anthony R
Clementel Veronica
Buckell Jenny
Chapple Susan Dj
Clarke Kay F
Conquer Jennie S
Crofts Anna M
Crowther Sandra R E
Dyson Michael R
Flack Gillian
Griffin Gareth J
Hooks Yvette
Howat William J
Kolb-Kokocinski Anja
Kunze Susan
Martin Cecile D
Maslen Gareth L
Mitchell Joanne N
O'Sullivan Maureen
Perera Rajika L
Roake Wendy
Shadbolt S Paul
Vincent Karen J
Warford Anthony
Wilson Wendy E
Xie Jane
Young Joyce L
McCafferty John
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2007-8-11-r254
Email
darren.schofield@abcam.com
Published
2007-00-00
Pages
R254
Language
English
Region
England
NLM ID
100960660
PMCID
PMC2258204
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1186/gb-2007-8-11-r254 PMC Full Text View journal details
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