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PMID: 18042715 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 49 ·2007-12-04 ·Pages 19416-21

Yasui DH, Peddada S, Bieda MC, Vallero RO, Hogart A, Nagarajan RP, Thatcher KN, Farnham PJ, Lasalle JM

Abstract

Mutations in MECP2 cause the autism-spectrum disorder Rett syndrome. MeCP2 is predicted to bind to methylated promoters and silence transcription. However, the first large-scale mapping of neuronal MeCP2-binding sites on 26.3 Mb of imprinted and nonimprinted loci revealed that 59% of MeCP2-binding sites are outside of genes and that only 6% are in CpG islands. Integrated genome-wide promoter analysis of MeCP2 binding, CpG methylation, and gene expression revealed that 63% of MeCP2-bound promoters are actively expressed and that only 6% are highly methylated. These results indicate that the primary function of MeCP2 is not the silencing of methylated promoters.

MeSH Terms
Binding Sites Chromatin Immunoprecipitation CpG Islands DNA Methylation Gene Expression Regulation Gene Silencing Humans Methyl-CpG-Binding Protein 2/genetics,metabolism Neurons/metabolism Promoter Regions, Genetic Rett Syndrome/genetics
Chemicals
Methyl-CpG-Binding Protein 2
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Yasui Dag H
Department of Medical Microbiology and Immunology, Rowe Program in Human Genetics, School of Medicine, University of California, 1 Shields Avenue, Davis, CA 95616, USA.
Peddada Sailaja
Bieda Mark C
Vallero Roxanne O
Hogart Amber
Nagarajan Raman P
Thatcher Karen N
Farnham Peggy J
Lasalle Janine M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0707442104
Published
2007-12-04
Epub
2007-00-27
Pages
19416-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2148304
Subset
IM
Grants
NICHD NIH HHS · R01 HD041462-05 · United States
NINDS NIH HHS · R01 NS041462 · United States
NICHD NIH HHS · R01 HD048799-03 · United States
NCRR NIH HHS · C06 RR12088 · United States
NICHD NIH HHS · R01 HD/NS41462 · United States
NICHD NIH HHS · R01 HD041462-04 · United States
NCRR NIH HHS · C06 RR012088 · United States
NICHD NIH HHS · R01 HD048799 · United States
NICHD NIH HHS · R01 HD041462 · United States
Databases
GEO
GSE9568
External Links
PubMed source DOI: 10.1073/pnas.0707442104 PMC Full Text View journal details
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