Home LiteratureArticle Details
PMID: 18039863 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II.

Molecular and cellular biology ·Vol. 28 ·No. 3 ·2008-02-00 ·Pages 1041-6

Smith ER, Lee MG, Winter B, Droz NM, Eissenberg JC, Shiekhattar R, Shilatifard A

Abstract

Histone H3 methylation at Lys27 (H3K27 methylation) is a hallmark of silent chromatin, while H3K4 methylation is associated with active chromatin regions. Here we report that a Drosophila JmjC family member, dUTX, specifically demethylates di- and trimethylated but not monomethylated H3K27. dUTX localization on chromatin correlates with the elongating form of RNA polymerase II (Pol II), and dUTX can associate with Pol II. Furthermore, heat shock induction results in the recruitment of dUTX to the hsp70 gene, like that of several other Pol II elongation factors. Our data indicate that dUTX is intimately associated with actively transcribed genes and may provide a paradigm for how H3K27 demethylation is required for the activation of preinitiated Pol II on transcriptionally poised genes.

MeSH Terms
Animals Chromatin/metabolism Drosophila Proteins/metabolism Female HSP70 Heat-Shock Proteins/genetics Histones/metabolism Male Methylation Nuclear Proteins/metabolism Oxidoreductases, N-Demethylating/metabolism RNA Polymerase II/metabolism Transcription, Genetic
Chemicals
Chromatin Drosophila Proteins HSP70 Heat-Shock Proteins Histones Nuclear Proteins Oxidoreductases, N-Demethylating UTX protein, Drosophila RNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Smith Edwin R
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
Lee Min Gyu
Winter Benjamin
Droz Nathan M
Eissenberg Joel C
Shiekhattar Ramin
Shilatifard Ali
References (32)
32 references, click to expand
  1. Drosophila Paf1 modulates chromatin structure at actively transcribed genes.
    Mol Cell Biol. 2006 Jan;26(1):250-60 PMID: 16354696
  2. Linking global histone acetylation to the transcription enhancement of X-chromosomal genes in Drosophila males.
    J Biol Chem. 2001 Aug 24;276(34):31483-6 PMID: 11445559
  3. Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination.
    Science. 2007 Oct 19;318(5849):447-50 PMID: 17761849
  4. Regulation of heat shock gene expression by RNA polymerase II elongation factor, Elongin A.
    J Biol Chem. 2005 Feb 11;280(6):4017-20 PMID: 15611125
  5. Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock.
    Mol Cell Biol. 2003 Nov;23(21):7628-37 PMID: 14560008
  6. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing.
    Cell. 2007 Sep 21;130(6):1083-94 PMID: 17825402
  7. Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein.
    Cell. 2007 Mar 9;128(5):877-87 PMID: 17320162
  8. A chromatin landmark and transcription initiation at most promoters in human cells.
    Cell. 2007 Jul 13;130(1):77-88 PMID: 17632057
  9. JMJD3 is a histone H3K27 demethylase.
    Cell Res. 2007 Oct;17(10):850-7 PMID: 17923864
  10. Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains.
    Genes Dev. 2003 Aug 1;17(15):1870-81 PMID: 12897054
  11. The trithorax-group protein Lid is a histone H3 trimethyl-Lys4 demethylase.
    Nat Struct Mol Biol. 2007 Apr;14(4):341-3 PMID: 17351631
  12. The UTX gene escapes X inactivation in mice and humans.
    Hum Mol Genet. 1998 Apr;7(4):737-42 PMID: 9499428
  13. Regulation of histone methylation by demethylimination and demethylation.
    Nat Rev Mol Cell Biol. 2007 Apr;8(4):307-18 PMID: 17342184
  14. Histone demethylation by hydroxylation: chemistry in action.
    ACS Chem Biol. 2006 Mar 17;1(2):75-81 PMID: 17163647
  15. Drosophila Rtf1 functions in histone methylation, gene expression, and Notch signaling.
    Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11970-4 PMID: 16882721
  16. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development.
    Nature. 2007 Oct 11;449(7163):731-4 PMID: 17713478
  17. RNA polymerase II: just stopping by.
    Cell. 2007 Jul 13;130(1):16-8 PMID: 17632051
  18. The drosophila MSL complex acetylates histone H4 at lysine 16, a chromatin modification linked to dosage compensation.
    Mol Cell Biol. 2000 Jan;20(1):312-8 PMID: 10594033
  19. A histone H3 lysine 27 demethylase regulates animal posterior development.
    Nature. 2007 Oct 11;449(7163):689-94 PMID: 17851529
  20. Functional genomics of histone modification and non-histone chromosomal proteins using the polytene chromosomes of Drosophila.
    Methods. 2006 Dec;40(4):360-4 PMID: 17101449
  21. Epigenetic regulation of cellular memory by the Polycomb and Trithorax group proteins.
    Annu Rev Genet. 2004;38:413-43 PMID: 15568982
  22. Chromatin modifications by methylation and ubiquitination: implications in the regulation of gene expression.
    Annu Rev Biochem. 2006;75:243-69 PMID: 16756492
  23. High-resolution profiling of histone methylations in the human genome.
    Cell. 2007 May 18;129(4):823-37 PMID: 17512414
  24. The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase.
    Nat Struct Mol Biol. 2007 Apr;14(4):344-6 PMID: 17351630
  25. Drosophila ELL is associated with actively elongating RNA polymerase II on transcriptionally active sites in vivo.
    EMBO J. 2001 Nov 1;20(21):6104-14 PMID: 11689450
  26. The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth.
    Genes Dev. 2007 Mar 1;21(5):537-51 PMID: 17311883
  27. Histone lysine demethylases: emerging roles in development, physiology and disease.
    Nat Rev Genet. 2007 Nov;8(11):829-33 PMID: 17909537
  28. Mammalian male mutation bias: impacts of generation time and regional variation in substitution rates.
    J Mol Evol. 2006 Oct;63(4):537-44 PMID: 16955237
  29. Knockdown of ALR (MLL2) reveals ALR target genes and leads to alterations in cell adhesion and growth.
    Mol Cell Biol. 2007 Mar;27(5):1889-903 PMID: 17178841
  30. SMRT-mediated repression of an H3K27 demethylase in progression from neural stem cell to neuron.
    Nature. 2007 Nov 15;450(7168):415-9 PMID: 17928865
  31. TPR proteins: the versatile helix.
    Trends Biochem Sci. 2003 Dec;28(12):655-62 PMID: 14659697
  32. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
    J Biol Chem. 2007 Jul 13;282(28):20395-406 PMID: 17500065
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01504-07
Published
2008-02-00
Epub
2007-00-26
Pages
1041-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2223382
Subset
IM
Grants
NIGMS NIH HHS · GM61204 · United States
NCI NIH HHS · R01 CA089455 · United States
NCI NIH HHS · CA089455 · United States
NIGMS NIH HHS · GM069905 · United States
NIGMS NIH HHS · R01 GM069905 · United States
NIGMS NIH HHS · R01 GM061204 · United States
External Links
PubMed source DOI: 10.1128/MCB.01504-07 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2