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PMID: 18034892 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells.

Molecular cancer ·Vol. 6 ·2007-11-22 ·Pages 75

Seo DC, Sung JM, Cho HJ, Yi H, Seo KH, Choi IS, Kim DK, Kim JS, El-Aty AM A, Shin HC

Abstract

The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells. We isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non-side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip(R) oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (AKR1C1/C2), Transmembrane 4 L six family member 1 nuclear receptor (TM4SF1), and Nuclear receptor subfamily 0, group B, member 1 (NR0B1) were significantly up-regulated in SP compared to non-SP cells. This is the first report indicating the differences of gene expression pattern between SP and non-SP cells in A549 cells. We suggest that the up-regulations of the genes AKR1C1/C2, TM4SF1 and NR0B1 in SP of human adenocarcinoma A549 cells could be a target of poor prognosis in anti-cancer therapy.

MeSH Terms
Adenocarcinoma/genetics,metabolism,pathology Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Lung Neoplasms/genetics,metabolism,pathology Neoplasm Proteins/genetics,metabolism Neoplastic Stem Cells/metabolism,pathology Oligonucleotide Array Sequence Analysis/methods RNA, Messenger/genetics,metabolism RNA, Neoplasm/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Neoplasm Proteins RNA, Messenger RNA, Neoplasm
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Seo Dong-Cheol
Department of Veterinary Pharmacology and Toxicology College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea. sdc97@hanmail.net
Sung Ji-Min
Cho Hee-Jung
Yi Hee
Seo Kun-Ho
Choi In-Soo
Kim Dong-Ku
Kim Jin-Suk
El-Aty AM Abd
Shin Ho-Chul
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Article Info
Journal
Molecular cancer
Abbr.
Mol Cancer
ISSN
1476-4598
DOI
10.1186/1476-4598-6-75
Email
sdc97@hanmail.net
Published
2007-11-22
Epub
2007-00-22
Pages
75
Language
English
Region
England
NLM ID
101147698
PMCID
PMC2234429
Subset
IM
External Links
PubMed source DOI: 10.1186/1476-4598-6-75 PMC Full Text View journal details
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