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PMID: 18030492 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SAZ, a new SUPERMAN-like protein, negatively regulates a subset of ABA-responsive genes in Arabidopsis.

Molecular genetics and genomics : MGG ·Vol. 279 ·No. 2 ·2008-02-00 ·Pages 183-92

Jiang CJ, Aono M, Tamaoki M, Maeda S, Sugano S, Mori M, Takatsuji H

Abstract

Arabidopsis SUPERMAN (SUP) and members of its family are plant-unique C(2)H(2)-type zinc finger genes that have been implicated in plant growth and development. In this paper, we report that a new SUP-family gene, designated as S A- and A BA-downregulated z inc finger gene (SAZ), is involved in the negative regulation of ABA-mediated signaling. SAZ-GUS fusion proteins were predominantly localized in the nuclei when they were transiently expressed in onion epidermal cells. SAZ transcripts were expressed in the leaves and pistils of very young flower buds. In young seedlings, SAZ expression was downregulated in response to environmental stresses such as drought, salt, ozone and ultraviolet-B irradiation. This downregulation was also observed in response to the phytohormones salicylic acid (SA) and abscisic acid (ABA). SA-responsive downregulation of SAZ was not observed in the npr1-1 mutant, indicating that this regulation is NPR1 dependent. RNAi-mediated knockdown of SAZ (SAZ-kd) resulted in elevated expression of the drought- and ABA-responsive genes rd29B and rab18 under unstressed conditions, and it enhanced the response of these genes to drought and ABA treatment. The expression of several other drought- and/or ABA-responsive genes was not affected by SAZ-kd. Based on these results, we propose that SAZ plays a role in repressing a subset of the ABA-mediated stress-responsive genes in unstressed conditions.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins/genetics Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Genotype Glucuronidase/genetics,metabolism Models, Genetic Molecular Sequence Data Phenotype Plant Growth Regulators/pharmacology Plants, Genetically Modified RNA Interference RNA, Plant/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Transcription Factors/genetics Zinc Fingers/genetics
Chemicals
Arabidopsis Proteins Plant Growth Regulators RNA, Plant Recombinant Fusion Proteins Transcription Factors superman protein, Arabidopsis Abscisic Acid Glucuronidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jiang Chang-Jie
Plant Disease Resistance Research Unit, Division of Plant Science, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, Japan.
Aono Mitsuko
Tamaoki Masanori
Maeda Satoru
Sugano Shoji
Mori Masaki
Takatsuji Hiroshi
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-007-0306-1
Published
2008-02-00
Epub
2007-00-21
Pages
183-92
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
Databases
GENBANK
BT010828, BT011301
External Links
PubMed source DOI: 10.1007/s00438-007-0306-1 View journal details
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