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PMID: 18025306 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin.

The Journal of cell biology ·Vol. 179 ·No. 4 ·2007-11-19 ·Pages 733-46

Ilani T, Khanna C, Zhou M, Veenstra TD, Bretscher A

Abstract

Immunological synapse (IS) formation involves receptor-ligand pair clustering and intracellular signaling molecule recruitment with a coincident removal of other membrane proteins away from the IS. As microfilament-membrane linkage is critical to this process, we investigated the involvement of ezrin and moesin, the two ezrin/radixin/moesin proteins expressed in T cells. We demonstrate that ezrin and moesin, which are generally believed to be functionally redundant, are differentially localized and have important and complementary functions in IS formation. Specifically, we find that ezrin directly interacts with and recruits the signaling kinase ZAP-70 to the IS. Furthermore, the activation of ezrin by phosphorylation is essential for this process. In contrast, moesin dephosphorylation and removal, along with CD43, are necessary to prepare a region of the cell cortex for IS. Thus, ezrin and moesin have distinct and critical functions in the T cell cortex during IS formation.

MeSH Terms
Animals Calcium/metabolism Cytoskeletal Proteins/genetics,metabolism,physiology Humans Jurkat Cells Leukosialin/metabolism,physiology Lymphocyte Activation/immunology Microfilament Proteins/genetics,metabolism,physiology Mutation Phosphorylation Precipitin Tests RNA, Small Interfering/metabolism Recombinant Proteins/metabolism Spodoptera/cytology,metabolism T-Lymphocytes/physiology Transfection ZAP-70 Protein-Tyrosine Kinase/metabolism,physiology
Chemicals
Cytoskeletal Proteins Leukosialin Microfilament Proteins RNA, Small Interfering Recombinant Proteins ezrin moesin ZAP-70 Protein-Tyrosine Kinase ZAP70 protein, human Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ilani Tal
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Khanna Chand
Zhou Ming
Veenstra Timothy D
Bretscher Anthony
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200707199
Published
2007-11-19
Pages
733-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2080902
Subset
IM
Grants
NCI NIH HHS · N01CO12400 · United States
NCI NIH HHS · N01-CO-12400 · United States
External Links
PubMed source DOI: 10.1083/jcb.200707199 PMC Full Text View journal details
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