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题目:: Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain.
作者:: Perier(Aurélie),Gourier(Christine),Pichard(Sylvain),Husson(Julien),Lajeunesse(Evelyne),Babon(Aurélie),Menez(André),Gillet(Daniel)
状态:: 发布时间2007-11-20 , 更新时间 2007-11-20
期刊:: FEBS Lett
摘要:: We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.
语言:: eng
DOI:: 10.1016/j.febslet.2007.10.055

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