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PMID: 17978175 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Duplication and segregation of the actin (MreB) cytoskeleton during the prokaryotic cell cycle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 45 ·2007-11-06 ·Pages 17795-800

Vats P, Rothfield L

Abstract

The bacterial actin homolog MreB exists as a single-copy helical cytoskeletal structure that extends between the two poles of rod-shaped bacteria. In this study, we show that equipartition of the MreB cytoskeleton into daughter cells is accomplished by division and segregation of the helical MreB array into two equivalent structures located in opposite halves of the predivisional cell. This process ensures that each daughter cell inherits one copy of the MreB cytoskeleton. The process is triggered by the membrane association of the FtsZ cell division protein. The cytoskeletal division and segregation events occur before and independently of cytokinesis and involve specialized MreB structures that appear to be intermediates in this process.

MeSH Terms
Cell Cycle Cell Division Cytoskeleton/physiology,ultrastructure Escherichia coli/cytology Escherichia coli Proteins/chemistry,genetics,physiology Image Processing, Computer-Assisted Microscopy, Fluorescence Models, Molecular Protein Conformation
Chemicals
Escherichia coli Proteins MreB protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vats Purva
Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA.
Rothfield Lawrence
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0708739104
Published
2007-11-06
Epub
2007-00-31
Pages
17795-800
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2077029
Subset
IM
Grants
NIGMS NIH HHS · GM R37-06032 · United States
External Links
PubMed source DOI: 10.1073/pnas.0708739104 PMC Full Text View journal details
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