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PMID: 17965723 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A charged and contoured surface on the nucleosome regulates chromatin compaction.

Nature structural & molecular biology ·Vol. 14 ·No. 11 ·2007-11-00 ·Pages 1105-7

Chodaparambil JV, Barbera AJ, Lu X, Kaye KM, Hansen JC, Luger K

Abstract

Local nucleosome-nucleosome interactions in cis drive chromatin folding, whereas interactions in trans lead to fiber-fiber oligomerization. Here we show that peptides derived from the histone H4 tail and Kaposi's sarcoma herpesvirus LANA protein can replace the endogenous H4 tail, resulting in array folding and oligomerization. Neutralization of a LANA binding site on the histone surface enhanced rather than abolished nucleosome-nucleosome interactions. We maintain that the contoured nucleosome surface is centrally involved in regulating chromatin condensation.

MeSH Terms
Antigens, Viral/chemistry,genetics,metabolism Chromatin/chemistry,metabolism Chromatin Assembly and Disassembly Histones/chemistry,genetics,metabolism Humans Macromolecular Substances/chemistry,metabolism Nuclear Proteins/chemistry,genetics,metabolism Nucleic Acid Conformation Nucleosomes/chemistry,metabolism Peptides/chemistry,genetics,metabolism Protein Conformation Protein Folding Surface Properties
Chemicals
Antigens, Viral Chromatin Histones Macromolecular Substances Nuclear Proteins Nucleosomes Peptides latency-associated nuclear antigen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chodaparambil Jayanth V
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870, USA.
Barbera Andrew J
Lu Xu
Kaye Kenneth M
Hansen Jeffrey C
Luger Karolin
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb1334
Published
2007-11-00
Epub
2007-00-28
Pages
1105-7
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2366819
Subset
IM
Grants
NIGMS NIH HHS · R01 GM045916-18 · United States
NIGMS NIH HHS · GM45916 · United States
PHS HHS · HHMI_SCHNIZER-LUGER_K · United States
Howard Hughes Medical Institute · United States
NCI NIH HHS · R01 CA082036 · United States
NIGMS NIH HHS · GM067777 · United States
NCI NIH HHS · R01 CA082036-07 · United States
NIGMS NIH HHS · R01 GM067777 · United States
NIGMS NIH HHS · R01 GM045916 · United States
NCI NIH HHS · CA82036 · United States
NIGMS NIH HHS · R01 GM067777-04 · United States
Corrections
CommentIn
PMID: 17984968
External Links
PubMed source DOI: 10.1038/nsmb1334 PMC Full Text View journal details
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