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PMID: 17965719 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Two-photon photostimulation and imaging of neural circuits.

Nature methods ·Vol. 4 ·No. 11 ·2007-11-00 ·Pages 943-50

Nikolenko V, Poskanzer KE, Yuste R

Abstract

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

MeSH Terms
Acetates/chemistry Action Potentials/drug effects Animals Brain Mapping/instrumentation,methods Calcium Signaling/drug effects Cytophotometry/instrumentation,methods Electrophysiology Excitatory Postsynaptic Potentials/drug effects Glutamates/metabolism Glutamine/pharmacology Indoles/chemistry,metabolism Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Fluorescence, Multiphoton/instrumentation,methods Neocortex/cytology,drug effects,physiology Nerve Net/cytology,metabolism,physiology Neurons/cytology,drug effects,physiology Reproducibility of Results Tetrodotoxin/pharmacology
Chemicals
4-methoxy-7-nitroindolinyl-glutamate Acetates Glutamates Indoles mag-indo-1 AM Glutamine Indo-1 pentaacetoxymethyl ester Tetrodotoxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nikolenko Volodymyr
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, Box 2435, New York, New York 10027, USA. vn59@columbia.edu
Poskanzer Kira E
Yuste Rafael
Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7091
DOI
10.1038/nmeth1105
Email
vn59@columbia.edu
Published
2007-11-00
Epub
2007-00-28
Pages
943-50
Language
English
Region
United States
NLM ID
101215604
Subset
IM
External Links
PubMed source DOI: 10.1038/nmeth1105 View journal details
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