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PMID: 17956130 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association.

Biochemistry ·Vol. 46 ·No. 45 ·2007-11-13 ·Pages 13041-8

Fuson KL, Montes M, Robert JJ, Sutton RB

Abstract

Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction has been lacking. The 2.7 A crystal structure of the cytosolic domains of human synaptotagmin 1 in the absence of Ca2+ reveals a novel closed conformation of the protein. The shared interface between C2A and C2B is stabilized by a network of interactions between residues on the C-terminal alpha-helix of the C2B domain and residues on loops 1-3 of the Ca2+-binding region of C2A. These interactions alter the overall shape of the Ca2+-binding pocket of C2A, but not that of C2B. Thus, synaptotagmin 1 C2A-C2B may utilize a novel regulatory mechanism whereby one C2 domain could regulate the other until an appropriate triggering event decouples them.

MeSH Terms
Amino Acid Sequence Calcium/chemistry Humans Models, Molecular Molecular Sequence Data Protein Conformation Protein Structure, Tertiary Sequence Alignment Synaptotagmin I/chemistry
Chemicals
Synaptotagmin I Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fuson Kerry L
Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas 77555, USA.
Montes Miguel
Robert J Justin
Sutton R Bryan
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Article Info
Journal
Biochemistry
Abbr.
Biochemistry
ISSN
0006-2960
Published
2007-11-13
Epub
2007-00-23
Pages
13041-8
Language
English
Region
United States
NLM ID
0370623
PMCID
PMC5975968
Subset
IM
Grants
Wellcome Trust · United Kingdom
NIMH NIH HHS · R21 MH070589 · United States
NIGMS NIH HHS · T32 GM008280 · United States
NIMH NIH HHS · MH-070589 · United States
Databases
PDB
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