Home LiteratureArticle Details
PMID: 17942559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent.

Journal of virology ·Vol. 82 ·No. 1 ·2008-01-00 ·Pages 569-74

Molina S, Castet V, Pichard-Garcia L, Wychowski C, Meurs E, Pascussi JM, Sureau C, Fabre JM, Sacunha A, Larrey D, Dubuisson J, Coste J, McKeating J, Maurel P, Fournier-Wirth C

Abstract

Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

MeSH Terms
Adolescent Adult Aged Antigens, CD/genetics,immunology,physiology Cells, Cultured Female Gene Silencing Hepacivirus/physiology Hepatocytes/chemistry,virology Humans Male Middle Aged RNA, Viral/analysis Receptors, Virus/antagonists & inhibitors,genetics,immunology,physiology Tetraspanin 28 Virus Internalization
Chemicals
Antigens, CD CD81 protein, human RNA, Viral Receptors, Virus Tetraspanin 28
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Molina Sonia
Inserm U632, Hepatic Physiopathology, 1919 route de Mende, 34293 Montpellier, Cedex 5, France.
Castet Valerie
Pichard-Garcia Lydiane
Wychowski Czeslaw
Meurs Eliane
Pascussi Jean-Marc
Sureau Camille
Fabre Jean-Michel
Sacunha Antonio
Larrey Dominique
Dubuisson Jean
Coste Joliette
McKeating Jane
Maurel Patrick
Fournier-Wirth Chantal
References (46)
46 references, click to expand
  1. Identification of amino acid residues in CD81 critical for interaction with hepatitis C virus envelope glycoprotein E2.
    J Virol. 2000 Apr;74(8):3642-9 PMID: 10729140
  2. Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR.
    J Virol. 1995 Dec;69(12):8079-83 PMID: 7494326
  3. Basic statistical considerations in virological experiments.
    J Virol. 2005 Jan;79(2):669-76 PMID: 15613294
  4. Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus.
    Hepatology. 2005 Feb;41(2):265-74 PMID: 15660396
  5. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR.
    Virology. 1994 Aug 1;202(2):606-14 PMID: 8030225
  6. Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry.
    Nature. 2007 Apr 12;446(7137):801-5 PMID: 17325668
  7. Extraordinarily low density of hepatitis C virus estimated by sucrose density gradient centrifugation and the polymerase chain reaction.
    J Gen Virol. 1992 Mar;73 ( Pt 3):715-8 PMID: 1312126
  8. The role of the nuclear receptor CAR as a coordinate regulator of hepatic gene expression in defense against chemical toxicity.
    Arch Biochem Biophys. 2003 Jan 1;409(1):207-11 PMID: 12464260
  9. Density heterogeneities of hepatitis C virus in human sera due to the binding of beta-lipoproteins and immunoglobulins.
    Med Microbiol Immunol. 1993 Dec;182(6):329-34 PMID: 8121333
  10. Secretion of functional plasma haemostasis proteins in long-term primary cultures of human hepatocytes.
    Br J Haematol. 2004 Jun;125(5):638-46 PMID: 15147380
  11. CD81 is an entry coreceptor for hepatitis C virus.
    Proc Natl Acad Sci U S A. 2004 May 11;101(19):7270-4 PMID: 15123813
  12. Diverse CD81 proteins support hepatitis C virus infection.
    J Virol. 2006 Nov;80(22):11331-42 PMID: 16943299
  13. Characterization of low- and very-low-density hepatitis C virus RNA-containing particles.
    J Virol. 2002 Jul;76(14):6919-28 PMID: 12072493
  14. Transcriptional regulation of CYP2C9 gene. Role of glucocorticoid receptor and constitutive androstane receptor.
    J Biol Chem. 2002 Jan 4;277(1):209-17 PMID: 11679585
  15. The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus.
    EMBO J. 2002 Oct 1;21(19):5017-25 PMID: 12356718
  16. Characterisation of the differences between hepatitis C virus genotype 3 and 1 glycoproteins.
    J Med Virol. 2003 Jul;70(3):361-72 PMID: 12766998
  17. DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2.
    J Biol Chem. 2003 May 30;278(22):20358-66 PMID: 12609975
  18. Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles.
    Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7271-6 PMID: 12761383
  19. Binding of the hepatitis C virus E2 glycoprotein to CD81 is strain specific and is modulated by a complex interplay between hypervariable regions 1 and 2.
    J Virol. 2003 Feb;77(3):1856-67 PMID: 12525620
  20. Role of N glycosylation of hepatitis B virus envelope proteins in morphogenesis and infectivity of hepatitis delta virus.
    J Virol. 2003 May;77(9):5519-23 PMID: 12692255
  21. Human hepatocyte culture.
    Methods Mol Biol. 2006;320:283-93 PMID: 16719400
  22. Long-term primary cultures of adult human hepatocytes.
    Chem Biol Interact. 1997 Nov 6;107(1-2):31-45 PMID: 9402948
  23. Identification of host genes involved in hepatitis C virus replication by small interfering RNA technology.
    Hepatology. 2007 Jun;45(6):1413-21 PMID: 17518369
  24. Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor.
    J Biol Chem. 2003 Oct 24;278(43):41624-30 PMID: 12913001
  25. In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus.
    J Gen Virol. 1998 Oct;79 ( Pt 10):2367-74 PMID: 9780041
  26. Myristoylation signal transfer from the large to the middle or the small HBV envelope protein leads to a loss of HDV particles infectivity.
    Virology. 2007 Aug 15;365(1):204-9 PMID: 17462692
  27. Expression of CYP3A4, CYP2B6, and CYP2C9 is regulated by the vitamin D receptor pathway in primary human hepatocytes.
    J Biol Chem. 2002 Jul 12;277(28):25125-32 PMID: 11991950
  28. Complete replication of hepatitis C virus in cell culture.
    Science. 2005 Jul 22;309(5734):623-6 PMID: 15947137
  29. Possible involvement of pregnane X receptor-enhanced CYP24 expression in drug-induced osteomalacia.
    J Clin Invest. 2005 Jan;115(1):177-86 PMID: 15630458
  30. Density analysis of hepatitis C virus particle population in the circulation of infected hosts: implications for virus neutralization or persistence.
    J Hepatol. 1995 Apr;22(4):440-8 PMID: 7665862
  31. Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5.
    Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):183-8 PMID: 12518064
  32. Use of long-term cultures of human hepatocytes to study cytochrome P450 gene expression.
    Methods Enzymol. 2002;357:311-21 PMID: 12424921
  33. Oxidized low-density lipoprotein inhibits hepatitis C virus cell entry in human hepatoma cells.
    Hepatology. 2006 May;43(5):932-42 PMID: 16628670
  34. Interleukin 1beta inhibits CAR-induced expression of hepatic genes involved in drug and bilirubin clearance.
    Hepatology. 2004 Oct;40(4):951-60 PMID: 15382119
  35. Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes.
    J Exp Med. 2003 Mar 3;197(5):633-42 PMID: 12615904
  36. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.
    Nat Med. 2005 Jul;11(7):791-6 PMID: 15951748
  37. Viral and cellular determinants of the hepatitis C virus envelope-heparan sulfate interaction.
    J Virol. 2006 Nov;80(21):10579-90 PMID: 16928753
  38. Characterization of hepatitis C virus (HCV) and HCV E2 interactions with CD81 and the low-density lipoprotein receptor.
    J Virol. 2000 Nov;74(21):10055-62 PMID: 11024134
  39. Structure-function analysis of hepatitis C virus envelope-CD81 binding.
    J Virol. 2000 May;74(10):4824-30 PMID: 10775621
  40. Role of the asialoglycoprotein receptor in binding and entry of hepatitis C virus structural proteins in cultured human hepatocytes.
    J Virol. 2003 Jan;77(1):546-59 PMID: 12477859
  41. Robust hepatitis C virus infection in vitro.
    Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9294-9 PMID: 15939869
  42. Alpha interferon inhibits hepatitis C virus replication in primary human hepatocytes infected in vitro.
    J Virol. 2002 Aug;76(16):8189-99 PMID: 12134024
  43. Binding of hepatitis C virus to CD81.
    Science. 1998 Oct 30;282(5390):938-41 PMID: 9794763
  44. Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate.
    J Biol Chem. 2003 Oct 17;278(42):41003-12 PMID: 12867431
  45. CD81 engineered with endocytotic signals mediates HCV cell entry: implications for receptor usage by HCV in vivo.
    Virology. 2003 Apr 10;308(2):250-69 PMID: 12706076
  46. The low-density lipoprotein receptor plays a role in the infection of primary human hepatocytes by hepatitis C virus.
    J Hepatol. 2007 Mar;46(3):411-9 PMID: 17156886
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.01443-07
Published
2008-01-00
Epub
2007-00-17
Pages
569-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2224376
Subset
IM
Grants
Medical Research Council · G0400802 · United Kingdom
Medical Research Council · G0801976 · United Kingdom
External Links
PubMed source DOI: 10.1128/JVI.01443-07 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2