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PMID: 17936912 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Physiological glucose is critical for optimized neuronal viability and AMPK responsiveness in vitro.

Journal of neuroscience methods ·Vol. 167 ·No. 2 ·2008-01-30 ·Pages 292-301

Kleman AM, Yuan JY, Aja S, Ronnett GV, Landree LE

Abstract

Understanding the mechanisms that govern neuronal responses to oxidative and metabolic stress is essential for therapeutic intervention. In vitro modeling is an important approach for these studies, as the metabolic environment influences neuronal responses. Surprisingly, most neuronal culture methods employ conditions that are non-physiological, especially with regards to glucose concentrations, which often exceed 20mM. This concentration is a significant departure from physiological glucose levels, and even several-fold greater than that seen during severe hyperglycemia. The goal of this study was to establish a physiological neuronal culture system that will facilitate the study of neuronal energy metabolism and responses to metabolic stress. We demonstrate that the metabolic environment during preparation, plating, and maintenance of cultures affects neuronal viability and the response of neuronal pathways to changes in energy balance.

MeSH Terms
4-Butyrolactone/analogs & derivatives,pharmacology AMP-Activated Protein Kinases Adenosine Triphosphate/metabolism Aminoimidazole Carboxamide/analogs & derivatives,pharmacology Analysis of Variance Animals Cell Survival Cells, Cultured Cerebral Cortex/cytology Dose-Response Relationship, Drug Embryo, Mammalian Glucose/administration & dosage,metabolism Hypoglycemic Agents/pharmacology Multienzyme Complexes/metabolism Neurons/drug effects,physiology Protein Serine-Threonine Kinases/metabolism Rats Rats, Sprague-Dawley Ribonucleotides/pharmacology Time Factors
Chemicals
4-methylene-2-octyl-5-oxofuran-3-carboxylic acid Hypoglycemic Agents Multienzyme Complexes Ribonucleotides Aminoimidazole Carboxamide Adenosine Triphosphate Protein Serine-Threonine Kinases AMP-Activated Protein Kinases AICA ribonucleotide Glucose 4-Butyrolactone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kleman Amy M
Department of Neuroscience, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
Yuan Jason Y
Aja Susan
Ronnett Gabriele V
Landree Leslie E
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Article Info
Journal
Journal of neuroscience methods
Abbr.
J Neurosci Methods
ISSN
0165-0270
DOI
10.1016/j.jneumeth.2007.08.028
Published
2008-01-30
Epub
2007-00-07
Pages
292-301
Language
English
Region
Netherlands
NLM ID
7905558
PMCID
PMC2257477
Subset
IM
Grants
NINDS NIH HHS · R01 NS050625 · United States
NINDS NIH HHS · R01 NS050625-03 · United States
NINDS NIH HHS · R01 NS050625-02 · United States
NINDS NIH HHS · R01 NS050625-04 · United States
NINDS NIH HHS · R01 NS050625-01 · United States
External Links
PubMed source DOI: 10.1016/j.jneumeth.2007.08.028 PMC Full Text View journal details
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