Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.

Nucleic acids research ·Vol. 3 ·No. 3 ·1976-03-00 ·Pages 661-76

Rosenthal LJ

Abstract

During late lytic infection of mouse kidney cell cultures polyoma 16S and 19S (late 19S RNA) were isolated by oligo(dT)-cellulose chromatography. Approximately 60-80% of total cytoplasmic polyoma RNA contained tracts of poly(A) which were retained by oligo(dT)-cellulose. Early in lytic infection when viral DNA synthesis and the production of capsid protein are blocked by the addition of 5-fluorodeoxyuridine, approximately 100% of polyoma "early" 19S RNA was quantitatively retained by oligo(dT)-cellulose indicating the presence of poly(A) tracts on most 19S mRNA molecules. In addition, 2 classes polyoma RNA, synthesized after the onset of cellular RNA synthesis under conditions where DNA synthesis is inhibited with 5-fluorodeoxyuridine, were found to contain tracts of poly(A). These species sedimenting at 16S and 19S in aqueous sucrose density gradients were also quantitatively retained by oligo (dT)-cellulose.

MeSH Terms

Cell Line Cell Transformation, Neoplastic DNA Replication/drug effects Floxuridine/pharmacology Nucleic Acid Hybridization Poly A/analysis Polyomavirus RNA, Messenger/biosynthesis,isolation & purification RNA, Neoplasm/biosynthesis,isolation & purification RNA, Viral/biosynthesis,isolation & purification

Chemicals

RNA, Messenger RNA, Neoplasm RNA, Viral Floxuridine Poly A

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Rosenthal L J

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25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.3.661

Published

1976-03-00

Pages

661-76

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342932

Subset

External Links

PubMed source DOI: 10.1093/nar/3.3.661 PMC Full Text View journal details

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Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.

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