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PMID: 17892534 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A bioinformatics approach to identifying tail-anchored proteins in the human genome.

Traffic (Copenhagen, Denmark) ·Vol. 8 ·No. 12 ·2007-12-00 ·Pages 1687-1694

Kalbfleisch T, Cambon A, Wattenberg BW

Abstract

Intracellular proteins with a carboxy-terminal transmembrane domain and the amino-terminus oriented toward the cytosol are known as 'tail-anchored' proteins. Tail-anchored proteins have been of considerable interest because several important classes of proteins, including the vesicle-targeting/fusion proteins known as SNAREs and the apoptosis-related proteins of the Bcl-2 family, among others, utilize this unique membrane-anchoring motif. Here, we use a bioinformatic technique to develop a comprehensive list of potentially tail-anchored proteins in the human genome. Our final list contains 411 entries derived from 325 unique genes. We also analyzed both known and predicted tail-anchored proteins with respect to the amino acid composition of the transmembrane segments. This analysis revealed a distinctive composition of the membrane anchor in SNARE proteins.

MeSH Terms
Amino Acids/chemistry Cell Membrane/metabolism Computational Biology/methods Cytosol/metabolism Endoplasmic Reticulum/metabolism Genome, Human HeLa Cells Humans Intracellular Membranes/metabolism Membrane Proteins/metabolism Microscopy, Fluorescence Mitochondria/metabolism Protein Structure, Tertiary Proteomics/methods Proto-Oncogene Proteins c-bcl-2/metabolism
Chemicals
Amino Acids Membrane Proteins Proto-Oncogene Proteins c-bcl-2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kalbfleisch Ted
Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA. | Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA.
Cambon Alex
Department of Bioinformatics and Biostatistics, School of Public Health and Information Sciences, University of Louisville, Louisville, KY 40202, USA.
Wattenberg Binks W
Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA. | Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA. | Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USA.
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28 references, click to expand
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Article Info
Journal
Traffic (Copenhagen, Denmark)
Abbr.
Traffic
ISSN
1398-9219
DOI
10.1111/j.1600-0854.2007.00661.x
Published
2007-12-00
Epub
2007-00-17
Pages
1687-1694
Language
English
Region
England
NLM ID
100939340
Subset
IM
Grants
PHS HHS · 18733 · United States
NIEHS NIH HHS · 1P30ES014443-01A1 · United States
NCI NIH HHS · R01CA111987 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0854.2007.00661.x View journal details
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