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PMID: 17890362 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New properties of Drosophila fab-7 insulator.

Genetics ·Vol. 177 ·No. 1 ·2007-09-00 ·Pages 113-21

Rodin S, Kyrchanova O, Pomerantseva E, Parshikov A, Georgiev P

Abstract

In the Abd-B 3' cis-regulatory region, which is subdivided into a series of iab domains, boundary elements have previously been detected, including the Fab-7 element providing for the autonomous functioning of the iab-6 and iab-7 cis-regulatory domains. Here, it has been shown that a single copy of the 860-bp Fab-7 insulator effectively blocks the yellow and white enhancers. The eye and testis enhancers can stimulate the white promoter across the pair of Fab-7, which is indicative of a functional interaction between the insulators. Unexpectedly, Fab-7 has proved to lose the enhancer-blocking activity when placed near the white promoter. It seems likely that Fab-7 strengthens the relatively weak white promoter, which leads to the efficient enhancer-promoter interaction and insulator bypass.

MeSH Terms
ATP-Binding Cassette Transporters/genetics Animals Animals, Genetically Modified Drosophila Proteins/genetics Drosophila melanogaster/embryology,genetics Enhancer Elements, Genetic/genetics Eye/cytology,metabolism Eye Proteins/genetics Female Gene Expression Regulation/genetics Insulator Elements/genetics Male Promoter Regions, Genetic Transgenes/physiology
Chemicals
ATP-Binding Cassette Transporters Drosophila Proteins Eye Proteins w protein, Drosophila y protein, Drosophila
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rodin Sergey
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
Kyrchanova Olga
Pomerantseva Ekaterina
Parshikov Alexander
Georgiev Pavel
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.107.075887
Published
2007-09-00
Pages
113-21
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2013716
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.107.075887 PMC Full Text View journal details
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