Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range gram-negative cosmid vector pJRD215.

Molecular microbiology ·Vol. 5 ·No. 6 ·1991-06-00 ·Pages 1561-6

Hermans J, Martin C, Huijberts GN, Goosen T, de Bont JA

Abstract

The transformation of Mycobacterium aurum and Mycobacterium smegmatis with the Gram-negative RSF1010-derived cosmid pJRD215 is described. The plasmid is stably maintained in both species and the antibiotic resistance determinants for kanamycin and streptomycin are expressed. Southern blot analysis shows that rearrangements take place both in M. aurum and in M. smegmatis. The use of pJRD215 in mycobacterial cloning systems is discussed.

MeSH Terms

Blotting, Southern Cloning, Molecular Cosmids/genetics Drug Resistance, Microbial/genetics Electric Stimulation Gene Expression Kanamycin Resistance/genetics Mycobacterium/drug effects,genetics Recombination, Genetic/genetics Restriction Mapping Streptomycin/pharmacology Transformation, Genetic/genetics

Chemicals

Streptomycin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Hermans J

Department of Food Science, Agricultural University, Wageningen, The Netherlands.

Martin C

Huijberts G N

Goosen T

de Bont J A

Article Info

Journal

Molecular microbiology

Abbr.

Mol Microbiol

ISSN

0950-382X

DOI

10.1111/j.1365-2958.1991.tb00803.x

Published

1991-06-00

Pages

1561-6

Language

English

Region

England

NLM ID

8712028

Subset

External Links

PubMed source DOI: 10.1111/j.1365-2958.1991.tb00803.x View journal details

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Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range gram-negative cosmid vector pJRD215.

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