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PMID: 17873883 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The structure of bacterial ParM filaments.

Nature structural & molecular biology ·Vol. 14 ·No. 10 ·2007-10-00 ·Pages 921-6

Orlova A, Garner EC, Galkin VE, Heuser J, Mullins RD, Egelman EH

Abstract

Bacterial ParM is a homolog of eukaryotic actin and is involved in moving plasmids so that they segregate properly during cell division. Using cryo-EM and three-dimensional reconstruction, we show that ParM filaments have a different structure from F-actin, with very different subunit-subunit interfaces. These interfaces result in the helical handedness of the ParM filament being opposite to that of F-actin. Like F-actin, ParM filaments have a variable twist, and we show that this involves domain-domain rotations within the ParM subunit. The present results yield new insights into polymorphisms within F-actin, as well as the evolution of polymer families.

MeSH Terms
Actins/genetics,metabolism,ultrastructure Cryoelectron Microscopy Escherichia coli/chemistry Escherichia coli Proteins/genetics,metabolism,ultrastructure Models, Molecular Molecular Sequence Data Protein Structure, Quaternary Protein Subunits/chemistry,genetics,metabolism
Chemicals
Actins Escherichia coli Proteins ParM protein, E coli Protein Subunits
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Orlova Albina
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908-0733, USA.
Garner Ethan C
Galkin Vitold E
Heuser John
Mullins R Dyche
Egelman Edward H
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9993
DOI
10.1038/nsmb1300
Published
2007-10-00
Epub
2007-00-16
Pages
921-6
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC3541950
Subset
IM
Grants
NIGMS NIH HHS · R01 GM075287-02 · United States
NIGMS NIH HHS · R01 GM075287 · United States
NIGMS NIH HHS · GM061010 · United States
NIGMS NIH HHS · GM675287 · United States
NIGMS NIH HHS · R01 GM061010 · United States
NIGMS NIH HHS · R01 GM061010-08 · United States
NIGMS NIH HHS · GM081303 · United States
NIGMS NIH HHS · R01 GM081303 · United States
Databases
PDB
2QU4
External Links
PubMed source DOI: 10.1038/nsmb1300 PMC Full Text View journal details
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