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PMID: 17827032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Imaging of a beta-peptide distribution in whole-body mice sections by MALDI mass spectrometry.

Journal of the American Society for Mass Spectrometry ·Vol. 18 ·No. 11 ·2007-11-00 ·Pages 1921-4

Stoeckli M, Staab D, Schweitzer A, Gardiner J, Seebach D

Abstract

Mass spectrometric imaging was applied to assess compound distributions on whole-body sections of mice after i.v. dosing of a beta-peptide and an alpha-peptide control. Animals were sacrificed at different time points (5 min, 1 h, 24 h) post-dose, providing simultaneous spatial as well as kinetic information. As a result of this study, no detection of the alpha-peptide control was observed at 1 h post-dose, while retention of the beta-peptide was observed for longer than 24 h post-dose.

MeSH Terms

Animals Image Processing, Computer-Assisted Kidney/metabolism Mice Mice, Transgenic Oligopeptides/analysis,pharmacokinetics Peptide Fragments/analysis,pharmacokinetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods Time Factors Tissue Distribution

Chemicals

Oligopeptides Peptide Fragments

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stoeckli Markus

Novartis Institutes for BioMedical Research, Basel, Switzerland. markus.stoeckli@novartis.com

Staab Dieter

Schweitzer Alain

Gardiner James

Seebach Dieter

References (9)

9 references, click to expand

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Article Info

Journal

Journal of the American Society for Mass Spectrometry

Abbr.

J Am Soc Mass Spectrom

ISSN

1044-0305

DOI

10.1016/j.jasms.2007.08.005

markus.stoeckli@novartis.com

Published

2007-11-00

Epub

2007-00-16

Pages

1921-4

Language

English

Region

United States

NLM ID

9010412

Subset

External Links

PubMed source DOI: 10.1016/j.jasms.2007.08.005 View journal details

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Imaging of a beta-peptide distribution in whole-body mice sections by MALDI mass spectrometry.

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