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PMID: 17824386 Published · ppublish English Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Sequencing of avian influenza virus genomes following random amplification.

BioTechniques ·Vol. 43 ·No. 2 ·2007-08-00 ·Pages 188, 190, 192

Afonso CL

Abstract

Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.

MeSH Terms

Animals Base Sequence Birds Chromosome Mapping/methods Genome, Viral/genetics Influenza A virus/genetics Influenza in Birds/virology Molecular Sequence Data Random Amplified Polymorphic DNA Technique/methods Sequence Analysis, DNA/methods

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Afonso Claudio L

U.S. Department of Agriculture, Athens, CA, USA. cafonso@seprl.usda.gov

Article Info

Journal

BioTechniques

Abbr.

Biotechniques

ISSN

0736-6205

DOI

10.2144/000112529

cafonso@seprl.usda.gov

Published

2007-08-00

Pages

188, 190, 192

Language

English

Region

England

NLM ID

8306785

Subset

External Links

PubMed source DOI: 10.2144/000112529 View journal details

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Sequencing of avian influenza virus genomes following random amplification.

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