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PMID: 17719611 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Mice lacking NKCC1 have normal olfactory sensitivity.

Physiology & behavior ·Vol. 93 ·No. 1-2 ·2008-01-28 ·Pages 44-9

Smith DW, Thach S, Marshall EL, Mendoza MG, Kleene SJ

Abstract

When olfactory receptor neurons respond to odors, a depolarizing Cl(-) efflux is a substantial part of the response. This requires that the resting neuron accumulate Cl(-) against an electrochemical gradient. In isolated olfactory receptor neurons, the Na(+)+K(+)+2Cl(-) cotransporter NKCC1 is essential for Cl(-) accumulation. However, in intact epithelium, a robust electrical olfactory response persists in mice lacking NKCC1. To determine whether NKCC1 is required for normal olfactory sensitivity, olfactory sensitivity was compared between knockout (KO) mice carrying a null mutation for NKCC1 and wild-type (WT) littermates. Using operant behavioral techniques, olfactory sensitivity was measured using a commercial liquid-dilution olfactometer. Detection thresholds for the simple odorants cineole, 1-heptanol, and 1-propanol were compared in KO and WT animals. Regardless of the stimulus conditions employed, no systematic differences in behavioral thresholds were evident between KO and WT animals. We conclude that NKCC1 is not required for normal olfactory sensitivity.

MeSH Terms
Animals Female Male Mice Mice, Inbred Strains Mice, Knockout Olfactory Mucosa/metabolism Olfactory Receptor Neurons/metabolism Sensory Thresholds/physiology Signal Transduction/physiology Smell/physiology Sodium-Potassium-Chloride Symporters/genetics,metabolism Solute Carrier Family 12, Member 2
Chemicals
Slc12a2 protein, mouse Sodium-Potassium-Chloride Symporters Solute Carrier Family 12, Member 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smith David W
Department of Psychology, University of Florida, Gainesville, FL 32611, United States.
Thach Sokunthirith
Marshall Erika L
Mendoza Mary-Grace
Kleene Steven J
References (34)
34 references, click to expand
  1. Thresholds for odor and nasal pungency.
    Physiol Behav. 1990 Nov;48(5):719-25 PMID: 2082372
  2. Chloride accumulation in mammalian olfactory sensory neurons.
    J Neurosci. 2004 Sep 8;24(36):7931-8 PMID: 15356206
  3. Functional expression of a mammalian odorant receptor.
    Science. 1998 Jan 9;279(5348):237-42 PMID: 9422698
  4. Mice deficient in G(olf) are anosmic.
    Neuron. 1998 Jan;20(1):69-81 PMID: 9459443
  5. Transduction mechanisms in vertebrate olfactory receptor cells.
    Physiol Rev. 1998 Apr;78(2):429-66 PMID: 9562035
  6. OMP gene deletion causes an elevation in behavioral threshold sensitivity.
    Neuroreport. 1999 Jan 18;10(1):15-9 PMID: 10094125
  7. Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter.
    Nat Genet. 1999 Jun;22(2):192-5 PMID: 10369265
  8. Mutation of the Na-K-Cl co-transporter gene Slc12a2 results in deafness in mice.
    Hum Mol Genet. 1999 Aug;8(8):1579-84 PMID: 10401008
  9. Mice lacking the basolateral Na-K-2Cl cotransporter have impaired epithelial chloride secretion and are profoundly deaf.
    J Biol Chem. 1999 Sep 17;274(38):26946-55 PMID: 10480906
  10. Mechanism of the excitatory Cl- response in mouse olfactory receptor neurons.
    Neuron. 2005 Feb 17;45(4):553-61 PMID: 15721241
  11. Deficits in sexual and aggressive behaviors in Cnga2 mutant mice.
    Nat Neurosci. 2005 Dec;8(12):1660-2 PMID: 16261133
  12. Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: a patch clamp analysis in gene-targeted mice.
    Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1970-5 PMID: 16446455
  13. Essential role of the main olfactory system in social recognition of major histocompatibility complex peptide ligands.
    J Neurosci. 2006 Feb 15;26(7):1961-70 PMID: 16481428
  14. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1.
    J Neurophysiol. 2006 Mar;95(3):2003-6 PMID: 16319203
  15. Pheromone detection in male mice depends on signaling through the type 3 adenylyl cyclase in the main olfactory epithelium.
    J Neurosci. 2006 Jul 12;26(28):7375-9 PMID: 16837584
  16. Visual detection, pattern discrimination and visual acuity in 14 strains of mice.
    Genes Brain Behav. 2006 Jul;5(5):389-403 PMID: 16879633
  17. Bestrophin-2 is a candidate calcium-activated chloride channel involved in olfactory transduction.
    Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12929-34 PMID: 16912113
  18. Loss of CNGB1 protein leads to olfactory dysfunction and subciliary cyclic nucleotide-gated channel trapping.
    J Biol Chem. 2006 Nov 17;281(46):35156-66 PMID: 16980309
  19. Pheromonal communication in vertebrates.
    Nature. 2006 Nov 16;444(7117):308-15 PMID: 17108955
  20. Olfactory discrimination ability of CD-1 mice for a large array of enantiomers.
    Neuroscience. 2007 Jan 5;144(1):295-301 PMID: 17045753
  21. Temporal development of cyclic nucleotide-gated and Ca2+ -activated Cl- currents in isolated mouse olfactory sensory neurons.
    J Neurophysiol. 2007 Jul;98(1):153-60 PMID: 17460108
  22. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium.
    J Physiol. 2007 Sep 15;583(Pt 3):1005-20 PMID: 17656441
  23. Performance of mice in an automated olfactometer: odor detection, discrimination and odor memory.
    Chem Senses. 1999 Dec;24(6):637-45 PMID: 10587496
  24. Disruption of the type III adenylyl cyclase gene leads to peripheral and behavioral anosmia in transgenic mice.
    Neuron. 2000 Sep;27(3):487-97 PMID: 11055432
  25. Ultrastructure of the inner ear of NKCC1-deficient mice.
    Hear Res. 2001 Jun;156(1-2):17-30 PMID: 11377879
  26. Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation.
    Science. 2001 Dec 7;294(5549):2172-5 PMID: 11739959
  27. Facilitation of calmodulin-mediated odor adaptation by cAMP-gated channel subunits.
    Science. 2001 Dec 7;294(5549):2176-8 PMID: 11739960
  28. Human and murine phenotypes associated with defects in cation-chloride cotransport.
    Annu Rev Physiol. 2002;64:803-43 PMID: 11826289
  29. Spontaneous versus reinforced olfactory discriminations.
    J Neurosci. 2002 Aug 15;22(16):6842-5 PMID: 12177181
  30. Importance of the CNGA4 channel gene for odor discrimination and adaptation in behaving mice.
    Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4299-304 PMID: 12649326
  31. Quantification of chemical vapors in chemosensory research.
    Chem Senses. 2003 Jul;28(6):467-77 PMID: 12907584
  32. The Ca-activated Cl channel and its control in rat olfactory receptor neurons.
    J Gen Physiol. 2003 Sep;122(3):349-63 PMID: 12939394
  33. Odors detected by mice deficient in cyclic nucleotide-gated channel subunit A2 stimulate the main olfactory system.
    J Neurosci. 2004 Apr 7;24(14):3703-10 PMID: 15071119
  34. General anosmia caused by a targeted disruption of the mouse olfactory cyclic nucleotide-gated cation channel.
    Neuron. 1996 Oct;17(4):681-93 PMID: 8893025
Article Info
Journal
Physiology & behavior
Abbr.
Physiol Behav
ISSN
0031-9384
DOI
10.1016/j.physbeh.2007.07.011
Published
2008-01-28
Epub
2007-00-01
Pages
44-9
Language
English
Region
United States
NLM ID
0151504
PMCID
PMC2257478
Subset
IM
Grants
NIDCD NIH HHS · R01 DC000926 · United States
NIDCD NIH HHS · R01 DC000926-12 · United States
NIDCD NIH HHS · R01 DC00926 · United States
External Links
PubMed source DOI: 10.1016/j.physbeh.2007.07.011 PMC Full Text View journal details
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