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PMID: 17693516 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Host protein binding and adhesive properties of H6 and H7 flagella of attaching and effacing Escherichia coli.

Journal of bacteriology ·Vol. 189 ·No. 20 ·2007-10-00 ·Pages 7426-35

Erdem AL, Avelino F, Xicohtencatl-Cortes J, Girón JA

Abstract

It had been suggested that the flagella of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) might contribute to host colonization. In this study, we set out to investigate the adhesive properties of H7 and H6 flagella. We studied the abilities of EHEC EDL933 (O157:H7) and EPEC E2348/69 (O127:H6) flagella to bind to bovine mucus, host proteins such as mucins, and extracellular matrix proteins. Through several approaches, we found that H6 and H7 flagella and their flagellin monomers bind to mucins I and II and to freshly isolated bovine mucus. A genetic approach showed that EHEC and EPEC fliC deletion mutants were significantly less adherent to bovine intestinal tissue than the parental wild-type strains. In addition, we found that EPEC bacteria and H6 flagella, but not EHEC, bound largely, in a dose-dependent manner, to collagen and to a lesser extent to laminin and fibronectin. We also report that EHEC O157:H7 strains agglutinate rabbit red blood cells via their flagella, a heretofore unknown phenotype in this pathogroup. Collectively, our data demonstrate that the H6 and H7 flagella possess adhesive properties, particularly the ability to bind mucins, that may contribute to colonization of mucosal surfaces.

MeSH Terms
Animals Bacterial Adhesion/physiology Cattle Cell Aggregation Erythrocytes/microbiology Escherichia coli/classification,physiology Escherichia coli Proteins/genetics Extracellular Matrix Proteins/metabolism Flagella/physiology Flagellin Gene Deletion Horses In Vitro Techniques Intestines/microbiology Mucins/metabolism Mucus/metabolism Rabbits Sheep
Chemicals
Escherichia coli Proteins Extracellular Matrix Proteins FliC protein, E coli Mucins Flagellin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Erdem Aysen L
Department of Immunobiology, University of Arizona, Tucson, AZ 85724, USA.
Avelino Fabiola
Xicohtencatl-Cortes Juan
Girón Jorge A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.00464-07
Published
2007-10-00
Epub
2007-00-10
Pages
7426-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2168434
Subset
IM
Grants
NIAID NIH HHS · R01 AI066012 · United States
NIAID NIH HHS · AI60211 · United States
NIAID NIH HHS · AI66012 · United States
External Links
PubMed source DOI: 10.1128/JB.00464-07 PMC Full Text View journal details
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