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题目:: Elevation of transforming growth factor beta 1 mRNA level in ES-D3 mouse embryonic stem cells cocultured with Balb/c3T3 A31 fibroblasts.
作者:: Katsube(K),Shimizu(N)
状态:: 发布时间1992-02-25 , 更新时间 2006-11-15
期刊:: Cell Struct Funct
摘要:: Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, Balb/c3T3 A31, the expression level of transforming growth factor beta 1 (TGF-beta 1) mRNA was elevated for 1 to 3 days. In a coculture of ES cells with primary embryonic fibroblasts, only slight accumulation of TGF-beta 1 mRNA was observed for 6 to 24 h and soon attenuated. In situ hybridization analysis revealed that TGF-beta 1 transcripts accumulated first in the masses of ES cells and subsequently in Balb/c3T3 A31 cells adjacent to ES cells' masses. The ability of ES cells to induce TGF-beta 1 in Balb/c3T3 A31 cells was not mediated by soluble factors and was lost upon differentiation. ES cells on primary embryonic fibroblasts grew in undifferentiated form, but those on Balb/c3T3 A31 cells stopped growing and formed embryoid cystic bodies. We suggest that TGF-beta 1 mRNA induction in a coculture is triggered by an interaction between differentiating ES cells and Balb/c3T3 A31, and that this activity is limited to undifferentiated ES cells.
语言:: eng
DOI:

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