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PMID: 1768115 Published · ppublish English Journal Article

Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.

Applied and environmental microbiology ·Vol. 57 ·No. 8 ·1991-08-00 ·Pages 2426-8

Chen JP, Nagayama F, Chang MC

Abstract

An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.

MeSH Terms
Aeromonas/enzymology,genetics Chitinases/genetics,metabolism Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology,genetics Restriction Mapping
Chemicals
Chitinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen J P
Council of Agriculture, Department of Food and Agriculture, Taipei, Republic of China.
Nagayama F
Chang M C
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20 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.57.8.2426-2428.1991
Published
1991-08-00
Pages
2426-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC183590
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.57.8.2426-2428.1991 PMC Full Text View journal details
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