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PMID: 17646934 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of singlet oxygen-induced apoptosis by cytosolic NADP+-dependent isocitrate dehydrogenase.

Molecular and cellular biochemistry ·Vol. 302 ·No. 1-2 ·2007-08-00 ·Pages 27-34

Kim SY, Lee SM, Tak JK, Choi KS, Kwon TK, Park JW

Abstract

Singlet oxygen is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules and it also promotes deleterious processes such as cell death. Recently, we demonstrated that the control of redox balance and the cellular defense against oxidative damage are the primary functions of cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) through supplying NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPc activity in HL-60 cells regulates singlet oxygen-induced apoptosis. When we examined the protective role of IDPc against singlet oxygen-induced apoptosis with HL-60 cells transfected with the cDNA for mouse IDPc in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPc expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen.

MeSH Terms
Animals Apoptosis/drug effects Apoptosis Regulatory Proteins/metabolism Cytosol/drug effects,enzymology HL-60 Cells Humans Immunoblotting Isocitrate Dehydrogenase/metabolism Membrane Potential, Mitochondrial/drug effects Mice Oxidation-Reduction/drug effects Singlet Oxygen/pharmacology Transfection
Chemicals
Apoptosis Regulatory Proteins Singlet Oxygen Isocitrate Dehydrogenase isocitrate dehydrogenase (NADP+)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kim Sun Yee
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, 702-701, Korea.
Lee Su Min
Tak Jean Kyoung
Choi Kyeong Sook
Kwon Taeg Kyu
Park Jeen-Woo
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/s11010-007-9421-x
Published
2007-08-00
Epub
2007-00-14
Pages
27-34
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/s11010-007-9421-x View journal details
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