Home LiteratureArticle Details
PMID: 17635225 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mesophyll conductance to CO2 in Arabidopsis thaliana.

The New phytologist ·Vol. 175 ·No. 3 ·2007-00-00 ·Pages 501-511

Flexas J, Ortuño MF, Ribas-Carbo M, Diaz-Espejo A, Flórez-Sarasa ID, Medrano H

Abstract

The close rosette growth form, short petioles and small leaves of Arabidopsis thaliana make measurements with commercial gas exchange cuvettes difficult. This difficulty can be overcome by growing A. thaliana plants in 'ice-cream cone-like' soil pots. This design permitted simultaneous gas exchange and chlorophyll fluorescence measurements from which the first estimates of mesophyll conductance to CO(2) (g(m)) in Arabidopsis were obtained and used to determine photosynthetic limitations during plant ageing from c. 30-45 d. Estimations of g(m) showed maximum values of 0.2 mol CO(2) m(-2) s(-1) bar(-1), lower than expected for a thin-leaved annual species. The parameterization of the response of net photosynthesis (A(N)) to chloroplast CO(2) concentrations (C(c)) yielded estimations of the maximum velocity of carboxylation (V(c,max_Cc)) which were also lower than those reported for other annual species. As A. thaliana plants aged from 30 to 45 d, there was a 40% decline of A(N) that was entirely the result of increased diffusional limitations to CO(2) transfer, with g(m) being the largest. The results suggest that in A. thaliana A(N) is limited by low g(m) and low capacity for carboxylation. Decreased g(m) is the main factor involved in early age-induced photosynthetic decline.

MeSH Terms
Arabidopsis/metabolism Carbon Dioxide/metabolism Chlorophyll/metabolism Fluorescence Photosynthesis Plant Leaves/cytology,metabolism
Chemicals
Chlorophyll Carbon Dioxide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Flexas J
Laboratori de Fisiologia Vegetal, Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Balears, Spain.
Ortuño M F
Departamento Botânica e Engenharia Biológica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Ribas-Carbo M
Laboratori de Fisiologia Vegetal, Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Balears, Spain.
Diaz-Espejo A
Instituto de Recursos Naturales y Agrobiología, CSIC, Apartado 1052, 41080 Sevilla, Spain.
Flórez-Sarasa I D
Laboratori de Fisiologia Vegetal, Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Balears, Spain.
Medrano H
Laboratori de Fisiologia Vegetal, Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Balears, Spain.
References (46)
46 references, click to expand
  1. Arabidopsis Genome Initiative. 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815.
  2. Bernacchi CJ, Portis AR, Nakano H, Von Caemmerer S, Long SP. 2002. Temperature response of mesophyll conductance. Implications for the determination of Rubisco enzyme kinetics and for limitations to photosynthesis in vivo. Plant Physiology 130: 1992-1998.
  3. Bernacchi CJ, Singsaas EL, Pimentel C, Portis AR, Long SP. 2001. Improved temperature response functions for models of Rubisco-limited photosynthesis. Plant, Cell & Environment 24: 253-259.
  4. Büssis D, Von Groll U, Fisahn J, Altmann T. 2006. Stomatal aperture can compensate altered stomatal density in Arabidopsis thaliana at growth light conditions. Functional Plant Biology 11: 1037-1043.
  5. Delfine S, Alvino A, Villani MC, Loreto F. 1999. Restrictions to carbon dioxide conductance and photosynthesis in spinach leaves recovering from salt stress. Plant Physiology 119: 1101-1106.
  6. Dodd AN, Parkinson K, Webb AAR. 2004. Independent circadian regulation of assimilation and stomatal conductance in the ztl-1 mutant of Arabidopsis. New Phytologist 162: 63-70.
  7. Donahue RA, Poulson ME, Edwards GE. 1997. A method for measuring whole plant photosynthesis in Arabidopsis thaliana. Photosynthesis Research 52: 263-269.
  8. Epstein E. 1972. Mineral nutrition of plants: principles and perspectives. New York, NY, USA: John Wiley.
  9. Ethier GH, Livingston NJ. 2004. On the need to incorporate sensitivity to CO2 transfer conductance into the Farquhar - von Caemmerer - Berry leaf photosynthesis model. Plant, Cell & Environment 27: 137-153.
  10. Ethier GH, Livingston NJ, Harrison DL, Black TA, Moran JA. 2006. Low stomatal and internal conductance to CO2 versus Rubisco deactivation as determinants of the photosynthetic decline of ageing evergreen leaves. Plant, Cell & Environment 29: 2168-2184.
  11. Evans JR, Loreto F. 2000. Acquisition and diffusion of CO2 in higher plant leaves. In: Leegood RC, Sharkey TD, Von Caemmerer S, eds. Photosynthesis: physiology and metabolism. Dordrecht, the Netherlands: Kluwer Academic Publishers, 321-351.
  12. Evans JR, Sharkey TD, Berry JA, Farquhar GD. 1986. Carbon isotope discrimination measured concurrently with gas exchange to investigate CO2 diffusion in leaves of higher plants. Australian Journal of Plant Physiology 13: 281-292.
  13. Evans JR, Von Caemmerer S. 1996. Carbon dioxide diffusion inside leaves. Plant Physiology 110: 339-346.
  14. Farquhar GD, Von Caemmerer S, Berry JA. 1980. A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149: 78-90.
  15. Flexas J, Bota J, Escalona JM, Sampol B, Medrano H. 2002. Effects of drought on photosynthesis in grapevines under field conditions: an evaluation of stomatal and mesophyll limitations. Functional Plant Biology 29: 461-471.
  16. Flexas J, Ribas-Carbó M, Hanson DT, Bota J, Otto B, Cifre J, McDowell N, Medrano H, Kaldenhoff R. 2006. Tobacco aquaporin NtAQP1 is involved in mesophyll conductance to CO2 in vivo. Plant Journal 48: 427-439.
  17. Friedrich JW, Huffaker RC. 1980. Photosynthesis, leaf resistance, and ribulose-1,5-bisphosphate carboxylase degradation in senescing barley leaves. Plant Physiology 65: 1103-1107.
  18. Galmés J, Medrano H, Flexas J. in press. Photosynthetic limitations in response to water stress and recovery in Mediterranean plants with different growth forms. New Phytologist.
  19. Genty B, Briantais JM, Baker NR. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta 990: 87-92.
  20. Grassi G, Magnani F. 2005. Stomatal, mesophyll conductance and biochemical limitations to photosynthesis as affected by drought and leaf ontogeny in ash and oak trees. Plant, Cell & Environment 28: 834-849.
  21. Hanba YT, Shibasaka M, Hayashi Y, Hayakawa T, Kasamo K, Terashima I, Katsuhara M. 2004. Overexpression of the barley aquaporin HvPIP2; 1 increases internal CO2 conductance and CO2 assimilation in the leaves of transgenic rice plants. Plant Cell Physiology 45: 521-529.
  22. Harley PC, Loreto F, Di Marco G, Sharkey TD. 1992. Theoretical considerations when estimating the mesophyll conductance to CO2 flux by the analysis of the response of photosynthesis to CO2. Plant Physiology 98: 1429-1436.
  23. Jones H. 1985. Partitioning stomatal and non-stomatal limitations to photosynthesis. Plant, Cell & Environment 8: 95-104.
  24. Laisk AK. 1977. Kinetics of photosynthesis and photorespiration in C3-plants. Moscow, Russia: Nauka. (In Russian.)
  25. Laisk A, Loreto F. 1996. Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluorescence. Ribulose-1,5-bisphosphate carboxylase/oxygenase specificity factor, dark respiration in the light, excitation distribution between photosystems, alternative electron transport rate, and mesophyll diffusion resistance. Plant Physiology 110: 903-912.
  26. Lake JA. 2004. Gas exchange: new challenges with Arabidopsis. New Phytologist 162: 1-3.
  27. Lascève G, Leymarie J, Vavasseur A. 1997. Alterations in light-induced stomatal opening in a starch-deficient mutant of Arabidopsis thaliana L. deficient in chloroplast phosphoglucomutase activity. Plant, Cell & Environment 20: 350-358.
  28. Long SP, Bernacchi CJ. 2003. Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error. Journal of Experimental Botany 54: 2393-2401.
  29. Loreto F, Di Marco G, Tricoli D, Sharkey TD. 1994. Measurements of mesophyll conductance, photosynthetic electron transport and alternative electron sinks of field grown wheat leaves. Photosynthesis Research 41: 397-403.
  30. Loreto F, Harley PC, Di Marco G, Sharkey TD. 1992. Estimation of mesophyll conductance to CO2 flux by three different methods. Plant Physiology 98: 1437-1443.
  31. Niinemets U, Cescatti A, Rodeghiero M, Tosens T. 2005. Leaf internal diffusion conductance limits photosynthesis more strongly in older leaves of Mediterranean evergreen broad-leaved species. Plant, Cell & Environment 28: 1552-1566.
  32. Piel C, Frank E, Le Roux X, Genty B. 2002. Effect of local irradiance on CO2 transfer conductance of mesophyll in walnut. Journal of Experimental Botany 53: 2423-2430.
  33. Poulson ME, Edwards GE, Browse J. 2002. Photosynthesis is limited at high leaf to air vapor pressure deficit in a mutant of Arabidopsis thaliana that lacks trienoic fatty acids. Photosynthesis Research 72: 55-63.
  34. Priault P, Vidal G, De Paepe R, Ribas-Carbo M. 2007. Leaf age-related changes in respiratory pathways are dependent on complex I activity in Nicotiana sylvestris. Physiologia Plantarum 129: 152-162.
  35. Ribas-Carbo M, Still C, Berry JA. 2002. Automated system for simultaneous analysis of δ13C, δ18O and CO2 concentration in small air samples. Rapid Communications in Mass Spectrometry 16: 339-345.
  36. Schultz HR. 1996. Leaf absorptance of visible radiation in Vitis vinifera L. estimates of age and shade effects with a simple field method. Scientia Horticulturae 66: 93-102.
  37. Singsaas EL, Ort DR, DeLucia EH. 2003. Elevated CO2 effects on mesophyll conductance and its consequences for interpreting photosynthetic physiology. Plant, Cell & Environment 27: 41-50.
  38. Sommerville CR, Ogren WL. 1982. Mutants of the cruciferous plant Arabidopsis thaliana lacking glycine decarboxylase activity. Biochemical Journal 202: 373-380.
  39. Syvertsen JP, Lloyd J, McConchie C, Kriedemann PE, Farquhar GD. 1995. On the relationship between leaf anatomy and CO2 diffusion through the mesophyll of hypostomatous leaves. Plant, Cell & Environment 18: 149-157.
  40. Tocquin P, Perilleux C. 2004. Design of a versatile device for measuring whole plant gas exchanges in Arabidopsis thaliana. New Phytologist 162: 223-229.
  41. Walters RG, Ibrahim DG, Horton P, Kruger NJ. 2004. A mutant of Arabidopsis lacking the triose-phosphate/phosphate translocator reveals metabolic regulation of starch breakdown in the light. Plant Physiology 135: 891-906.
  42. Warren CR. 2004. The photosynthetic limitation posed by internal conductance to CO2 movement is increased by nutrient supply. Journal of Experimental Botany 55: 2313-2321.
  43. Warren CR, Adams MA. 2006. Internal conductance does not scale with photosynthetic capacity: implications for carbon isotope discrimination and the economics of water and nitrogen use in photosynthesis. Plant, Cell & Environment 29: 192-201.
  44. Warren CR, Dreyer E. 2006. Temperature response of photosynthesis and internal conductance to CO2: results from two independent approaches. Journal of Experimental Botany 57: 3057-3067.
  45. Warren CR, Ethier GH, Livingston NJ, Grant NJ, Turpin DH, Harrison DL, Black TA. 2003. Transfer conductance in second growth Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) canopies. Plant, Cell & Environment 26: 1215-1227.
  46. Wullschleger SD. 1993. Biochemical limitations to carbon assimilation in C3 plants - a retrospective analysis of the A/Ci curves from 109 species. Journal of Experimental Botany 44: 907-920.
Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2007.02111.x
Published
2007-00-00
Pages
501-511
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1469-8137.2007.02111.x View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2