Abstract
The rad4.116 mutant of the fission yeast Schizosaccharomyces pombe is temperature-sensitive for growth, as well as being sensitive to the killing actions of both ultraviolet light and ionizing radiation. We have cloned the rad4 gene by complementation of the temperature sensitive phenotype of the rad4.116 mutant with a S. pombe gene bank. The rad4 gene fully complemented the UV sensitivity of the rad4.116 mutant. The gene is predicted to encode a protein of 579 amino acids with a basic tail, a possible zinc finger and a nuclear location signal. The amino terminal part of the predicted rad4 ORF contains two short regions of similarity to the C-terminal part of the human XRCC1 gene. Codon usage suggests that the gene is very poorly expressed, and this was confirmed by RNA studies. Gene disruption showed that the rad4 gene was essential for the mitotic growth of S. pombe.
MeSH Terms
Base Sequence
Blotting, Northern
Cloning, Molecular
Codon/genetics
DNA Repair/genetics,radiation effects
Gene Expression/genetics
Genes, Fungal/genetics
Genetic Complementation Test
Humans
Molecular Sequence Data
Open Reading Frames/genetics
Restriction Mapping
Schizosaccharomyces/genetics,radiation effects
Sequence Homology, Nucleic Acid
Temperature
Ultraviolet Rays
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fenech M
MRC Cell Mutation Unit, School of Biology, University of Sussex, Falmer, Brighton, UK.
Carr A M
Murray J
Watts F Z
Lehmann A R
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