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PMID: 17573535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis.

The Plant cell ·Vol. 19 ·No. 6 ·2007-06-00 ·Pages 2039-52

Nafisi M, Goregaoker S, Botanga CJ, Glawischnig E, Olsen CE, Halkier BA, Glazebrook J

Abstract

Camalexin (3-thiazol-2-yl-indole) is an indole alkaloid phytoalexin produced by Arabidopsis thaliana that is thought to be important for resistance to necrotrophic fungal pathogens, such as Alternaria brassicicola and Botrytis cinerea. It is produced from Trp, which is converted to indole acetaldoxime (IAOx) by the action of cytochrome P450 monooxygenases CYP79B2 and CYP79B3. The remaining biosynthetic steps are unknown except for the last step, which is conversion of dihydrocamalexic acid to camalexin by CYP71B15 (PAD3). This article reports characterization of CYP71A13. Plants carrying cyp71A13 mutations produce greatly reduced amounts of camalexin after infection by Pseudomonas syringae or A. brassicicola and are susceptible to A. brassicicola, as are pad3 and cyp79B2 cyp79B3 mutants. Expression levels of CYP71A13 and PAD3 are coregulated. CYP71A13 expressed in Escherichia coli converted IAOx to indole-3-acetonitrile (IAN). Expression of CYP79B2 and CYP71A13 in Nicotiana benthamiana resulted in conversion of Trp to IAN. Exogenously supplied IAN restored camalexin production in cyp71A13 mutant plants. Together, these results lead to the conclusion that CYP71A13 catalyzes the conversion of IAOx to IAN in camalexin synthesis and provide further support for the role of camalexin in resistance to A. brassicicola.

MeSH Terms
Alternaria/physiology Arabidopsis/enzymology,genetics,microbiology Arabidopsis Proteins/genetics,metabolism Carbon Monoxide/analysis Catalysis Cytochrome P-450 Enzyme System/metabolism DNA, Bacterial Gene Expression Regulation, Plant Genes, Plant Immunity, Innate Indoles/metabolism Mutagenesis, Insertional Oximes/metabolism Plant Diseases/immunology,microbiology Plant Leaves/microbiology Pseudomonas syringae/physiology RNA, Messenger/genetics,metabolism Recombinant Proteins/metabolism Substrate Specificity Thiazoles/metabolism Tobacco
Chemicals
Arabidopsis Proteins DNA, Bacterial Indoles Oximes RNA, Messenger Recombinant Proteins T-DNA Thiazoles camalexin indole-3-acetaldoxime Carbon Monoxide Cytochrome P-450 Enzyme System indole-3-acetonitrile cytochrome P-450 71A13 protein, Arabidopsis
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nafisi Majse
Plant Biochemistry Laboratory, Department of Plant Biology, Center for Molecular Plant Physiology, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Goregaoker Sameer
Botanga Christopher J
Glawischnig Erich
Olsen Carl E
Halkier Barbara A
Glazebrook Jane
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.051383
Published
2007-06-00
Epub
2007-00-15
Pages
2039-52
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1955726
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.107.051383 PMC Full Text View journal details
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