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PMID: 1756785 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Repetitive firing properties of putative dopamine-containing neurons in vitro: regulation by an apamin-sensitive Ca(2+)-activated K+ conductance.

Experimental brain research ·Vol. 86 ·No. 1 ·1991-00-00 ·Pages 141-50

Shepard PD, Bunney BS

Abstract

Intracellular recording techniques were used to study the effects of apamin (APA), a selective inhibitor of one type of Ca(2+)-activated K+ channel, on the electroresponsive properties of dopamine (DA)-containing neurons within the zona compacta of the substantia nigra (SNc) in rat. Bath application of APA (1 microM) blocked the slow component of a complex post-spike afterhyperpolarization (AHPs) without affecting other characteristics of the action potential. Blockade of AHPs was accompanied by an increase in the number and frequency of action potentials evoked by depolarizing current pulses. However, APA failed to affect the cellular mechanisms underlying spike frequency adaptation or post-stimulus inhibitory period. These data indicate that AHPs can exert a strong influence on the interspike interval but is probably not involved in regulating slower adaptive neuronal responses.

MeSH Terms
Action Potentials/drug effects,physiology Animals Apamin/pharmacology Calcium/physiology Cell Membrane/physiology Dopamine/metabolism Electrophysiology In Vitro Techniques Male Membrane Potentials/drug effects,physiology Microelectrodes Neurons/metabolism,physiology Potassium Channels/drug effects,physiology Rats Rats, Inbred Strains Substantia Nigra/cytology,drug effects,physiology
Chemicals
Potassium Channels Apamin Calcium Dopamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shepard P D
Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06510.
Bunney B S
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Article Info
Journal
Experimental brain research
Abbr.
Exp Brain Res
ISSN
0014-4819
DOI
10.1007/BF00231048
Published
1991-00-00
Pages
141-50
Language
English
Region
Germany
NLM ID
0043312
Subset
IM
Grants
NIMH NIH HHS · MH-14276 · United States
NIMH NIH HHS · MH-25642 · United States
NIMH NIH HHS · MH-28849 · United States
External Links
PubMed source DOI: 10.1007/BF00231048 View journal details
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