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PMID: 17548479 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Mutation of traP in Staphylococcus aureus has no impact on expression of agr or biofilm formation.

Infection and immunity ·Vol. 75 ·No. 9 ·2007-09-00 ·Pages 4528-33

Tsang LH, Daily ST, Weiss EC, Smeltzer MS

Abstract

To investigate the regulatory role of traP (target of RNAIII-activating peptide) in Staphylococcus aureus, we generated traP mutations in the clinical isolates UAMS-1 and USA300. In neither case did mutation of traP affect expression of the accessory gene regulator (agr) or the ability to form a biofilm. We were also unable to confirm that mutation of traP in the prototype 8325-4 laboratory strain RN6390 results in reduced expression of agr, reduced hemolytic activity, or an altered capacity to form a biofilm.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Biofilms/growth & development Gene Expression Regulation, Bacterial/physiology Mutagenesis RNA-Binding Proteins/genetics Staphylococcus aureus/genetics,physiology Trans-Activators/biosynthesis,genetics Transcription Factors/genetics
Chemicals
Agr protein, Staphylococcus aureus Bacterial Proteins MtrB protein, Bacteria RNA-Binding Proteins Trans-Activators Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tsang Laura H
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham, Mail Slot 511, Little Rock, AR 72205, USA.
Daily Sonja T
Weiss Elizabeth C
Smeltzer Mark S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.00603-07
Published
2007-09-00
Epub
2007-00-04
Pages
4528-33
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1951201
Subset
IM
Grants
NIAID NIH HHS · R13 AI074087 · United States
NIAID NIH HHS · AI074087 · United States
External Links
PubMed source DOI: 10.1128/IAI.00603-07 PMC Full Text View journal details
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