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PMID: 1754374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Liver specific transcription factors of the HNF3-, C/EBP- and LFB1-families interact with the A-activator binding site.

Nucleic acids research ·Vol. 19 ·No. 23 ·1991-12-11 ·Pages 6383-9

Drewes T, Klein-Hitpass L, Ryffel GU

Abstract

The A-activator binding site (AABS), present in the Xenopus A2 vitellogenin gene and several mammalian liver specifically expressed genes, interacts with different liver specific transcription factors including LFB1- and C/EBP-isobinders. We have now isolated some additional proteins interacting with AABS and show that they are HNF3-isobinders. The interactions between AABS and members of the HNF3 family are confirmed by binding studies using bacterially made HNF3-alpha protein. Thus a short DNA module of 24 bp is able to bind proteins of three different families of liver specific transcription factors. Competition experiments in the cell free in vitro transcription show that AABS dependent transcriptional activation is mediated by transcription factors belonging to at least two different families, the C/EBP- and the HNF3-isobinders. Being able to mediate the action of several distinct transactivators, AABS may thus be a prototype for a novel kind of tissue specific promoter modules with unique regulatory capacities.

MeSH Terms
Animals Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins DNA DNA-Binding Proteins/metabolism Electrophoresis, Polyacrylamide Gel Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Liver/metabolism Molecular Sequence Data Nuclear Proteins/metabolism Promoter Regions, Genetic Rats Transcription Factors/metabolism Transcription, Genetic Xenopus Xenopus Proteins
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Hepatocyte Nuclear Factor 1-alpha Hnf1a protein, rat Hnf1b protein, Xenopus Nuclear Proteins Transcription Factors Xenopus Proteins hnf1a protein, Xenopus Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-beta DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Drewes T
Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen, FRG.
Klein-Hitpass L
Ryffel G U
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.23.6383
Published
1991-12-11
Pages
6383-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329182
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.23.6383 PMC Full Text View journal details
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