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PMID: 17521566 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.

Neuron ·Vol. 54 ·No. 4 ·2007-05-24 ·Pages 511-33

Minor DL

Abstract

Structural biology now plays a prominent role in addressing questions central to understanding how excitable cells function. Although interest in the insights gained from the definition and dissection of macromolecular anatomy is high, many neurobiologists remain unfamiliar with the methods employed. This primer aims to help neurobiologists understand approaches for probing macromolecular structure and where the limits and challenges remain. Using examples of macromolecules with neurobiological importance, the review covers X-ray crystallography, electron microscopy (EM), small-angle X-ray scattering (SAXS), and nuclear magnetic resonance (NMR) and biophysical methods with which these approaches are often paired: isothermal titration calorimetry (ITC), equilibrium analytical ultracentifugation, and molecular dynamics (MD).

MeSH Terms
Animals Computational Biology Crystallography, X-Ray/methods Humans Magnetic Resonance Spectroscopy/methods Microscopy, Electron/methods Models, Molecular Molecular Structure Neurobiology/standards Structure-Activity Relationship
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Minor Daniel L
Cardiovascular Research Institute, Department of Biochemistry and Biophysics, California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158-2330, USA. daniel.minor@ucsf.edu
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/j.neuron.2007.04.026
Email
daniel.minor@ucsf.edu
Published
2007-05-24
Pages
511-33
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC3011226
Subset
IM
Grants
NINDS NIH HHS · R01 NS049272 · United States
NIDCD NIH HHS · R01 DC007664-03S1 · United States
NHLBI NIH HHS · R01 HL080050 · United States
NHLBI NIH HHS · R01 HL080050-03 · United States
NINDS NIH HHS · R01 NS049272-02 · United States
NIDCD NIH HHS · R01 DC007664 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2007.04.026 PMC Full Text View journal details
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