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PMID: 17507647 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Myosin Vb interacts with Rab8a on a tubular network containing EHD1 and EHD3.

Molecular biology of the cell ·Vol. 18 ·No. 8 ·2007-08-00 ·Pages 2828-37

Roland JT, Kenworthy AK, Peranen J, Caplan S, Goldenring JR

Abstract

Cells use multiple pathways to internalize and recycle cell surface components. Although Rab11a and Myosin Vb are involved in the recycling of proteins internalized by clathrin-mediated endocytosis, Rab8a has been implicated in nonclathrin-dependent endocytosis and recycling. By yeast two-hybrid assays, we have now demonstrated that Myosin Vb can interact with Rab8a, but not Rab8b. We have confirmed the interaction of Myosin Vb with Rab11a and Rab8a in vivo by using fluorescent resonant energy transfer techniques. Rab8a and Myosin Vb colocalize to a tubular network containing EHD1 and EHD3, which does not contain Rab11a. Myosin Vb tail can cause the accumulation of both Rab11a and Rab8a in collapsed membrane cisternae, whereas dominant-negative Rab11-FIP2(129-512) selectively accumulates Rab11a but not Rab8a. Additionally, dynamic live cell imaging demonstrates distinct pathways for Rab11a and Rab8a vesicle trafficking. These findings indicate that Rab8a and Rab11a define different recycling pathways that both use Myosin Vb.

MeSH Terms
Animals Carrier Proteins/metabolism Chickens Fluorescence Resonance Energy Transfer HeLa Cells Histocompatibility Antigens Class I/metabolism Humans Myosin Type V/metabolism Protein Binding Protein Transport Rabbits Transport Vesicles/metabolism Two-Hybrid System Techniques Vesicular Transport Proteins/metabolism rab GTP-Binding Proteins/metabolism
Chemicals
Carrier Proteins EHD1 protein, human EHD2 protein, human Histocompatibility Antigens Class I Vesicular Transport Proteins Myosin Type V rab11 protein RAB8A protein, human rab GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roland Joseph T
Department of Surgery, Vanderbilt University School of Medicine, Nashville, TN 37232-2733, USA.
Kenworthy Anne K
Peranen Johan
Caplan Steve
Goldenring James R
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e07-02-0169
Published
2007-08-00
Epub
2007-00-16
Pages
2828-37
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1949367
Subset
IM
Grants
NCI NIH HHS · CA-68485 · United States
NIDDK NIH HHS · DK-58404 · United States
NIDDK NIH HHS · R01 DK048370 · United States
NIDDK NIH HHS · R01DK-070856 · United States
NIDDK NIH HHS · P60 DK020593 · United States
NIGMS NIH HHS · R01 GM073846-03 · United States
NIGMS NIH HHS · R01 GM073846-01A1 · United States
NIDDK NIH HHS · F32DK-072789 · United States
NIDDK NIH HHS · P30 DK058404 · United States
NIGMS NIH HHS · R01GM-073846 · United States
NIDDK NIH HHS · DK-20593 · United States
NICHD NIH HHS · P30 HD015052 · United States
NICHD NIH HHS · HD-15052 · United States
NIDDK NIH HHS · F32 DK072789 · United States
NEI NIH HHS · P30 EY008126 · United States
NIDDK NIH HHS · P30 DK020593 · United States
NIDDK NIH HHS · R01 DK070856 · United States
NCI NIH HHS · P30 CA068485 · United States
NIGMS NIH HHS · R01 GM073846-02 · United States
NIGMS NIH HHS · R01GM-074877 · United States
NIGMS NIH HHS · R01 GM073846 · United States
NIDDK NIH HHS · DK-59637 · United States
NEI NIH HHS · EY-08126 · United States
NIDDK NIH HHS · R01DK-48370 · United States
NIDDK NIH HHS · U24 DK059637 · United States
External Links
PubMed source DOI: 10.1091/mbc.e07-02-0169 PMC Full Text View journal details
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