Abstract
The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters P(uspA), P(livJ), and P(rrnBP1) as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.
MeSH Terms
Amino Acids/metabolism
Bacterial Adhesion/genetics,physiology
Blotting, Western
Escherichia coli/genetics,metabolism,ultrastructure
Escherichia coli Proteins/genetics,metabolism,physiology
Gene Expression Regulation, Bacterial
Guanosine Tetraphosphate/metabolism,physiology
Microscopy, Electron, Scanning
Models, Biological
beta-Galactosidase/genetics,metabolism
Chemicals
Amino Acids
Escherichia coli Proteins
dksA protein, E coli
Guanosine Tetraphosphate
beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Magnusson Lisa U
Department of Cell and Molecular Biology-Microbiology, Göteborg University, Box 462, 405 30 Göteborg, Sweden. lisa.magnusson@gmm.gu.se
Gummesson Bertil
Joksimović Predrag
Farewell Anne
Nyström Thomas
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