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PMID: 17494708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interplay between laminar specificity and activity-dependent mechanisms of thalamocortical axon branching.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 27 ·No. 19 ·2007-05-09 ·Pages 5215-23

Uesaka N, Hayano Y, Yamada A, Yamamoto N

Abstract

Target and activity-dependent mechanisms of axonal branching were studied in the thalamocortical (TC) projection using organotypic cocultures of the thalamus and cortex. TC axons were labeled with enhanced yellow fluorescent protein (EYFP) by a single-cell electroporation method and observed over time by confocal microscopy. Changes in the firing activity of cocultures grown on multielectrode dishes were also monitored over time. EYFP-labeled TC axons exhibited more branch formation in and around layer 4 of the cortical explant during the second week in vitro, when spontaneous firing activity increased in both thalamic and cortical cells. Time-lapse imaging further demonstrated that branching patterns were generated dynamically by addition and elimination with a bias toward branch accumulation in the target layer. To examine the relationship between neural activity and TC branch formation, the dynamics of axonal branching was analyzed under various pharmacological treatments. Chronic blockade of firing or synaptic activity reduced the remodeling process, in particular, branch addition in the target layer. However, extension of branches was not affected by this treatment. Together, these findings suggest that neural activity can modify the molecular mechanisms that regulate lamina-specific TC axon branching.

MeSH Terms
Action Potentials/physiology Animals Animals, Newborn Bacterial Proteins/genetics Cell Communication Cell Differentiation/physiology Cells, Cultured Cerebral Cortex/cytology,embryology,physiology Coculture Techniques Cues Electroporation Excitatory Amino Acid Antagonists/pharmacology Growth Cones/physiology,ultrastructure Luminescent Proteins/genetics Microscopy, Confocal Neural Pathways/cytology,embryology,physiology Neuronal Plasticity/drug effects,physiology Organ Culture Techniques Rats Rats, Sprague-Dawley Sodium Channel Blockers/pharmacology Staining and Labeling/methods Synaptic Transmission/drug effects,physiology Thalamus/cytology,embryology,physiology
Chemicals
Bacterial Proteins Excitatory Amino Acid Antagonists Luminescent Proteins Sodium Channel Blockers yellow fluorescent protein, Bacteria
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Uesaka Naofumi
Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
Hayano Yasufumi
Yamada Akito
Yamamoto Nobuhiko
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.4685-06.2007
Published
2007-05-09
Pages
5215-23
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6672371
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.4685-06.2007 PMC Full Text View journal details
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