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PMID: 17485678 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation.

Tingley WG, Pawlikowska L, Zaroff JG, Kim T, Nguyen T, Young SG, Vranizan K, Kwok PY, Whooley MA, Conklin BR

Abstract

Sudden cardiac death due to abnormal heart rhythm kills 400,000-460,000 Americans each year. To identify genes that regulate heart rhythm, we are developing a screen that uses mouse embryonic stem cells (mESCs) with gene disruptions that can be differentiated into cardiac cells for phenotyping. Here, we show that the heterozygous disruption of the Akap10 (D-AKAP2) gene that disrupts the final 51 aa increases the contractile response of cultured cardiac cells to cholinergic signals. In both heterozygous and homozygous mutant mice derived from these mESCs, the same Akap10 disruption increases the cardiac response to cholinergic signals, suggesting a dominant interfering effect of the Akap10 mutant allele. The mutant mice have cardiac arrhythmias and die prematurely. We also found that a common variant of AKAP10 in humans (646V, 40% of alleles) was associated with increased basal heart rate and decreased heart rate variability (markers of low cholinergic/vagus nerve sensitivity). These markers predict an increased risk of sudden cardiac death. Although the molecular mechanism remains unknown, our findings in mutant mESCs, mice, and a common human AKAP10 SNP all suggest a role for AKAP10 in heart rhythm control. Our stem cell-based screen may provide a means of identifying other genes that control heart rhythm.

MeSH Terms
A Kinase Anchor Proteins Adaptor Proteins, Signal Transducing/chemistry,metabolism Amino Acid Sequence Animals Cells, Cultured Cholinergic Agonists/pharmacology Embryonic Stem Cells/drug effects,metabolism Female Genetics, Medical Genotype Heart Rate/drug effects,physiology Humans Male Mice Mice, Mutant Strains Middle Aged Molecular Sequence Data Mutation/genetics Myocytes, Cardiac/drug effects,metabolism Polymorphism, Single Nucleotide/genetics Signal Transduction/drug effects Survival Analysis Vagus Nerve/drug effects
Chemicals
A Kinase Anchor Proteins AKAP10 protein, human Adaptor Proteins, Signal Transducing Akap10 protein, mouse Cholinergic Agonists
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Tingley Whittemore G
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA.
Pawlikowska Ludmila
Zaroff Jonathan G
Kim Taeryn
Nguyen Trieu
Young Stephen G
Vranizan Karen
Kwok Pui-Yan
Whooley Mary A
Conklin Bruce R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
2007-05-15
Epub
2007-00-07
Pages
8461-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1866184
Subset
IM
Grants
NHLBI NIH HHS · HL66621 · United States
NHLBI NIH HHS · U01 HL066621 · United States
NCRR NIH HHS · RR18928 · United States
NHLBI NIH HHS · R01 HL060664 · United States
NHLBI NIH HHS · HL60664 · United States
NCRR NIH HHS · C06 RR018928 · United States
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