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PMID: 17483419 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Biochemical and genetic analyses provide insight into the structural and mechanistic properties of actin filament disassembly by the Aip1p cofilin complex in Saccharomyces cerevisiae.

Genetics ·Vol. 176 ·No. 3 ·2007-07-00 ·Pages 1527-39

Clark MG, Amberg DC

Abstract

Explication of the Aip1p/cofilin/actin filament complex may lead to a more detailed understanding of the mechanisms by which Aip1p and cofilin collaborate to rapidly disassemble filaments. We further characterized the actin-Aip1p interface through a random mutagenic screen of ACT1, identifying a novel Aip1p interaction site on actin. This finding is consistent with our current ternary complex model and offers insights into how Aip1p may disturb intersubunit contacts within an actin filament. In addition, site-directed mutagenesis aimed at interfering with salt bridge interactions at the predicted Aip1p-cofilin interface revealed hyperactive alleles of cof1 and aip1 that support the ternary complex model and suggest that conformational changes in cofilin structure may be transmitted to actin filaments, causing increased destabilization. Furthermore, these data support an active role for Aip1p in promoting actin filament turnover.

MeSH Terms
Actin Cytoskeleton/metabolism Binding Sites Cofilin 1/metabolism,physiology Microfilament Proteins/metabolism,physiology Multiprotein Complexes/metabolism,physiology Protein Conformation Protein Interaction Mapping Saccharomyces cerevisiae Proteins/metabolism,physiology
Chemicals
Cofilin 1 Microfilament Proteins Multiprotein Complexes Saccharomyces cerevisiae Proteins actin interacting protein 1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clark Michael G
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA.
Amberg David C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.107.072066
Published
2007-07-00
Epub
2007-00-04
Pages
1527-39
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1931519
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056189 · United States
NIGMS NIH HHS · GM-56189 · United States
External Links
PubMed source DOI: 10.1534/genetics.107.072066 PMC Full Text View journal details
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