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PMID: 17477556 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag.

Journal of proteome research ·Vol. 6 ·No. 6 ·2007-06-00 ·Pages 2057-67

Lemaire R, Stauber J, Wisztorski M, Van Camp C, Desmons A, Deschamps M, Proess G, Rudlof I, Woods AS, Day R, Salzet M, Fournier I

Abstract

MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.

MeSH Terms
Animals Antibodies/chemistry Brain Chemistry Mice Molecular Probes/chemistry Oligonucleotide Probes/chemistry Photolysis Proteins/analysis Proteome/genetics Proteomics/methods RNA, Messenger/analysis Rats Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods Transcription, Genetic
Chemicals
Antibodies Molecular Probes Oligonucleotide Probes Proteins Proteome RNA, Messenger
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Lemaire R
Laboratoire de Neuroimmunologie des Annélides, FRE CNRS 2933, MALDI Imaging Team, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Stauber J
Wisztorski M
Van Camp C
Desmons A
Deschamps M
Proess G
Rudlof I
Woods A S
Day R
Salzet M
Fournier I
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Article Info
Journal
Journal of proteome research
Abbr.
J Proteome Res
ISSN
1535-3893
DOI
10.1021/pr0700044
Published
2007-06-00
Epub
2007-00-04
Pages
2057-67
Language
English
Region
United States
NLM ID
101128775
PMCID
PMC2947822
Subset
IM
Grants
Intramural NIH HHS · Z99 DA999999 · United States
External Links
PubMed source DOI: 10.1021/pr0700044 PMC Full Text View journal details
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