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PMID: 17475776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner.

Molecular biology of the cell ·Vol. 18 ·No. 7 ·2007-07-00 ·Pages 2525-32

Chen SH, Wu PS, Chou CH, Yan YT, Liu H, Weng SY, Yang-Yen HF

Abstract

Translationally controlled Tumor Protein (TCTP) is an evolutionally highly conserved protein which has been implicated in many cellular functions that are related to cell growth, death, and even the allergic response of the host. To address the physiological roles of TCTP, we generated TCTP knockout mice by targeted gene disruption. Heterozygous mutants appeared to be developmentally normal. However, homozygous mutants (TCTP(-/-)) were embryonic lethal. TCTP(-/-) embryos were smaller in size than the control littermates at all postimplantation stages examined. Although TCTP is widely expressed in both extraembryonic and embryonic tissues, the most prominent defect of the TCTP(-/-) embryo at embryonic stage day 5.5 (E5.5) was in its epiblast, which had a reduced number of cells compared with wild-type controls. The knockout embryos also suffered a higher incidence of apoptosis in epiblast starting about E6.5 and subsequently died around E9.5-10.5 with a severely disorganized structure. Last, we demonstrated that TCTP(-/-) and control mouse embryonic fibroblasts manifested similar proliferation activities and apoptotic sensitivities to various death stimuli. Taken together, our results suggest that despite that TCTP is widely expressed in many tissues or cell types, it appears to regulate cell proliferation and survival in a tissue- or cell type-specific manner.

MeSH Terms

Animals Apoptosis Biomarkers, Tumor/deficiency,metabolism Cell Proliferation Cell Survival Cyclins/metabolism Embryo, Mammalian/abnormalities Female Fetal Death Fibroblasts/cytology G1 Phase G2 Phase Gastrula/metabolism Gene Targeting Male Mice Mice, Knockout Mitosis Mutation/genetics Organ Specificity Tumor Protein, Translationally-Controlled 1

Chemicals

Biomarkers, Tumor Cyclins Tpt1 protein, mouse Tumor Protein, Translationally-Controlled 1

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Chen Sung Ho

Institutes of Molecular Biology, Academia Sinica, Taipei, Taiwan.

Wu Peih-Shan

Chou Chiang-Hung

Yan Yu-Ting

Liu Hsuan

Weng Shih-Yen

Yang-Yen Hsin-Fang

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Article Info

Journal

Molecular biology of the cell

Abbr.

Mol Biol Cell

ISSN

1059-1524

DOI

10.1091/mbc.e07-02-0188

Published

2007-07-00

Epub

2007-00-02

Pages

2525-32

Language

English

Region

United States

NLM ID

9201390

PMCID

PMC1924818

Subset

External Links

PubMed source DOI: 10.1091/mbc.e07-02-0188 PMC Full Text View journal details

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A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner.

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