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PMID: 17429973 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

GM1 clustering inhibits cholera toxin binding in supported phospholipid membranes.

Journal of the American Chemical Society ·Vol. 129 ·No. 18 ·2007-05-09 ·Pages 5954-61

Shi J, Yang T, Kataoka S, Zhang Y, Diaz AJ, Cremer PS

Abstract

The present studies explore multivalent ligand-receptor interactions between pentameric cholera toxin B subunits (CTB) and the corresponding membrane ligand, ganglioside GM1. CTB binding was monitored on supported phospholipid bilayers coated on the walls and floors of microfluidic channels. Measurements were made by total internal reflection fluorescence microscopy (TIRFM). Apparent dissociation constants were extracted by fitting the binding data to both the Hill-Waud and Langmuir adsorption isotherm equations. Studies of the effect of ligand density on multivalent CTB-GM1 interactions revealed that binding weakened with increasing GM1 density from 0.02 mol % to 10.0 mol %. Such a result could be explained by the clustering of GM1 on the supported phospholipid membranes, which in turn inhibited the binding of CTB. Atomic force microscopy (AFM) experiments directly verified GM1 clustering within the supported POPC bilayers.

MeSH Terms
Cholera Toxin/metabolism G(M1) Ganglioside/metabolism Ligands Membrane Lipids/metabolism Microfluidics Phospholipids/metabolism Protein Binding
Chemicals
Ligands Membrane Lipids Phospholipids G(M1) Ganglioside Cholera Toxin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shi Jinjun
Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77843-3012, USA.
Yang Tinglu
Kataoka Sho
Zhang Yanjie
Diaz Arnaldo J
Cremer Paul S
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Article Info
Journal
Journal of the American Chemical Society
Abbr.
J Am Chem Soc
ISSN
0002-7863
DOI
10.1021/ja069375w
Published
2007-05-09
Epub
2007-00-13
Pages
5954-61
Language
English
Region
United States
NLM ID
7503056
PMCID
PMC3475181
Subset
IM
Grants
NIGMS NIH HHS · R01 GM070622 · United States
PHS HHS · R01-070622 · United States
External Links
PubMed source DOI: 10.1021/ja069375w PMC Full Text View journal details
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