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PMID: 17420280 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Alpha11 beta1 integrin-dependent regulation of periodontal ligament function in the erupting mouse incisor.

Molecular and cellular biology ·Vol. 27 ·No. 12 ·2007-06-00 ·Pages 4306-16

Popova SN, Barczyk M, Tiger CF, Beertsen W, Zigrino P, Aszodi A, Miosge N, Forsberg E, Gullberg D

Abstract

The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated a null allele of the alpha11 gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective incisors. Mutant incisors are characterized by disorganized periodontal ligaments, whereas molar ligaments appear normal. The primary defect in the incisor ligament leads to halted tooth eruption. alpha11beta1-defective embryonic fibroblasts displayed severe defects in vitro, characterized by (i) greatly reduced cell adhesion and spreading on collagen I, (ii) reduced ability to retract collagen lattices, and (iii) reduced cell proliferation. Analysis of matrix metalloproteinase in vitro and in vivo revealed disturbed MMP13 and MMP14 synthesis in alpha11(-/-) cells. We show that alpha11beta1 is the major receptor for collagen I on mouse embryonic fibroblasts and suggest that alpha11beta1 integrin is specifically required on periodontal ligament fibroblasts for cell migration and collagen reorganization to help generate the forces needed for axial tooth movement. Our data show a unique role for alpha11beta1 integrin during tooth eruption.

MeSH Terms
Animals Blastocyst Cell Line, Transformed Cell Movement Cell Transformation, Viral Cells, Cultured Collagen Type I/metabolism Collagen Type IV/metabolism Fibroblasts/cytology,metabolism Fibronectins/metabolism Gene Expression Regulation, Developmental/physiology Homozygote Immunohistochemistry Incisor/cytology,physiology Integrins/deficiency,genetics,physiology Mice Mice, Inbred C57BL Microinjections Periodontal Ligament/cytology,metabolism Receptors, Collagen/deficiency,genetics,physiology Tooth Eruption
Chemicals
Collagen Type I Collagen Type IV Fibronectins Integrins Receptors, Collagen integrin alpha11beta1
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Popova Svetlana N
Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway.
Barczyk Malgorzata
Tiger Carl-Fredrik
Beertsen Wouter
Zigrino Paola
Aszodi Attila
Miosge Nicolai
Forsberg Erik
Gullberg Donald
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00041-07
Published
2007-06-00
Epub
2007-00-09
Pages
4306-16
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1900066
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.00041-07 PMC Full Text View journal details
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