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题目:: Manipulation of cell-cell adhesion using bowtie-shaped microwells.
作者:: Nelson(Celeste M),Liu(Wendy F),Chen(Christopher S)
状态:: 发布时间2007-04-09 , 更新时间 2007-04-09
期刊:: Methods Mol Biol
摘要:: Traditional methods to study cell-cell adhesion have been limited by their inability to manipulate cell-cell interactions without simultaneously affecting other microenvironmental factors. Here we describe a novel method that enables the culture of cells with precise simultaneous control of both cell-cell and cell-substratum adhesion. Using microfabricated stamps of poly(dimethylsiloxane), we construct bowtie-shaped agarose microwells into which cells can be cultured. The degree to which cells spread is controlled by the size of the microwell; cell-cell contacts form between neighboring cells within the microwell. This chapter describes the details of stamp fabrication, agarose microwell construction, and cell culture in micropatterned substrata.
语言:: eng
DOI:: 10.1007/978-1-59745-353-0_1

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