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PMID: 17403776 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development.

Genes & development ·Vol. 21 ·No. 7 ·2007-04-01 ·Pages 744-9

Visvanathan J, Lee S, Lee B, Lee JW, Lee SK

Abstract

Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3' untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.

MeSH Terms
3' Untranslated Regions/metabolism Animals Avian Proteins/antagonists & inhibitors,genetics,metabolism Base Sequence Cells, Cultured Central Nervous System/embryology,enzymology,metabolism Chick Embryo Chickens Down-Regulation Humans Mice MicroRNAs/metabolism Molecular Sequence Data Neurons/enzymology,metabolism Phosphoprotein Phosphatases/antagonists & inhibitors,genetics,metabolism Protein Phosphatase 1 Repressor Proteins/antagonists & inhibitors Transcription Factors/antagonists & inhibitors Transfection
Chemicals
3' Untranslated Regions Avian Proteins MicroRNAs RE1-silencing transcription factor Repressor Proteins Transcription Factors Phosphoprotein Phosphatases Protein Phosphatase 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Visvanathan Jaya
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Lee Seunghee
Lee Bora
Lee Jae W
Lee Soo-Kyung
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1519107
Published
2007-04-01
Pages
744-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1838526
Subset
IM
Grants
NICHD NIH HHS · P30 HD024064 · United States
NINDS NIH HHS · R01 NS054941 · United States
NICHD NIH HHS · P30 HD24064 · United States
External Links
PubMed source DOI: 10.1101/gad.1519107 PMC Full Text View journal details
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