Home LiteratureArticle Details
PMID: 1740323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction.

Human genetics ·Vol. 88 ·No. 4 ·1992-02-00 ·Pages 457-62

Dunham I, Lengauer C, Cremer T, Featherstone T

Abstract

Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid generation of such probes using the polymerase chain reaction (PCR), and demonstrate their chromosome specificity by fluorescence in situ hybridization to normal human metaphase spreads and interphase nuclei. Oligonucleotide primers for conserved regions of the alpha satellite monomer were used to generate chromosome-specific DNA probes from somatic hybrid cells containing various human chromosomes, and from DNA libraries from sorted human chromosomes. Oligonucleotide primers for chromosome-specific regions of the alpha satellite monomer were used to generate specific DNA probes for the pericentromeric heterochromatin of human chromosomes 1, 6, 7, 17 and X directly from human genomic DNA.

MeSH Terms
Animals Base Sequence Cell Cycle Cell Line Chromosome Aberrations Chromosomes, Human Chromosomes, Human, Pair 1 Chromosomes, Human, Pair 17 Chromosomes, Human, Pair 6 Cricetinae DNA/genetics,isolation & purification DNA Probes Heterochromatin/physiology,ultrastructure Humans Metaphase Molecular Sequence Data Nucleic Acid Hybridization Oligodeoxyribonucleotides Polymerase Chain Reaction/methods X Chromosome
Chemicals
DNA Probes Heterochromatin Oligodeoxyribonucleotides DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dunham I
Department of Genetics, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110.
Lengauer C
Cremer T
Featherstone T
References (28)
28 references, click to expand
  1. Cytogenetic analysis by in situ hybridization with fluorescently labeled nucleic acid probes.
    Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:151-7 PMID: 3472711
  2. Reproducible compartmentalization of individual chromosome domains in human CNS cells revealed by in situ hybridization and three-dimensional reconstruction.
    Chromosoma. 1988;96(6):397-410 PMID: 3219911
  3. Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome.
    Nucleic Acids Res. 1985 Apr 25;13(8):2731-43 PMID: 2987865
  4. Interphase cytogenetics of hematological cancer: comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes.
    Cancer Res. 1991 Apr 1;51(7):1959-67 PMID: 2004382
  5. Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers.
    Hum Genet. 1990 Nov;86(1):1-6 PMID: 1701413
  6. Detection of numerical chromosome aberrations in bladder cancer by in situ hybridization.
    Am J Pathol. 1989 Dec;135(6):1105-17 PMID: 2688431
  7. Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84.
    Hum Genet. 1986 Dec;74(4):346-52 PMID: 3793097
  8. Characterization of human centromeric regions of specific chromosomes by means of alphoid DNA sequences.
    Am J Hum Genet. 1987 Sep;41(3):374-90 PMID: 3631075
  9. Double in situ hybridization in combination with digital image analysis: a new approach to study interphase chromosome topography.
    Exp Cell Res. 1989 Mar;181(1):126-40 PMID: 2917599
  10. Chromosome-specific subsets of human alpha satellite DNA: analysis of sequence divergence within and between chromosomal subsets and evidence for an ancestral pentameric repeat.
    J Mol Evol. 1987;25(3):207-14 PMID: 2822935
  11. Labeling of human centromeres using an alphoid DNA consensus sequence: application to the scoring of chromosome aberrations.
    Mutat Res. 1989 Jun;226(2):75-9 PMID: 2733720
  12. Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes.
    Exp Cell Res. 1988 Jun;176(2):199-220 PMID: 3288483
  13. Detection of numerical chromosomal abnormalities in neoplastic hematopoietic cells by in situ hybridization with a chromosome-specific probe.
    Am J Pathol. 1990 Jan;136(1):131-9 PMID: 2297044
  14. A cloned sequence, p82H, of the alphoid repeated DNA family found at the centromeres of all human chromosomes.
    Chromosoma. 1985;92(5):369-77 PMID: 2996845
  15. Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes.
    EMBO J. 1991 Jul;10(7):1629-34 PMID: 2050105
  16. Chromosomal in situ suppression hybridization after Giemsa banding.
    Hum Genet. 1991 Mar;86(5):484-6 PMID: 2016088
  17. The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion.
    Genomics. 1991 Apr;9(4):742-50 PMID: 2037299
  18. Chromosome-specific organization of human alpha satellite DNA.
    Am J Hum Genet. 1985 May;37(3):524-32 PMID: 2988334
  19. Chromosomal localization of complex and simple repeated human DNAs.
    Chromosoma. 1978 Mar 22;66(1):23-32 PMID: 639625
  20. Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ.
    Chromosoma. 1989 Oct;98(4):259-65 PMID: 2612286
  21. Highly reiterated sequences of SIMIANSIMIANSIMIANSIMIANSIMIAN.
    Science. 1978 Apr 28;200(4340):394-402 PMID: 205944
  22. Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1213-7 PMID: 2405397
  23. Second-generation approach to the construction of yeast artificial-chromosome libraries.
    Genomics. 1990 Oct;8(2):297-303 PMID: 2249850
  24. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633-7 PMID: 6273878
  25. Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.
    Hum Genet. 1988 Nov;80(3):235-46 PMID: 3192213
  26. Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome.
    Mol Cell Biol. 1986 Sep;6(9):3156-65 PMID: 3785225
  27. Distribution of chromosome 18 and X centric heterochromatin in the interphase nucleus of cultured human cells.
    Exp Cell Res. 1990 Jul;189(1):1-12 PMID: 2347371
  28. Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome.
    Mol Cell Biol. 1987 Jan;7(1):349-56 PMID: 3561394
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00215682
Published
1992-02-00
Pages
457-62
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00215682 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2