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题目:: Amplification and overexpression of Aurora-A in esophageal squamous cell carcinoma.
作者:: Yang(Shang-Bin),Zhou(Xiao-Bo),Zhu(Hong-Xia),Quan(Lan-Ping),Bai(Jin-Feng),He(Jie),Gao(Yan-Ning),Cheng(Shu-Jun),Xu(Ning-Zhi)
状态:: 发布时间2007-03-28 , 更新时间 2013-11-21
期刊:: Oncol Rep
摘要:: Aurora-A/BTAK/STK15 gene which encodes a centrosome-associated kinase is located on chromosome 20q13.2, a highly amplified region in various human tumors. Recent studies have demonstrated the overexpression and amplification of Aurora-A in many malignant human cancers. The purpose of this study was to investigate the amplification and expression of Aurora-A in esophageal squamous cell carcinoma. Amplification of Aurora-A was determined by fluorescence in situ hybridization in 7 esophageal cancer cell lines and real-time PCR in 29 esophageal cancer samples. We detected Aurora-A expression in 7 esophageal cancer cell lines and 38 esophageal cancers samples by semi-quantitative reverse transcription-PCR and Western blot hybridization. The amplification of Aurora-A was detected in 27 of 29 (93.1%) esophageal cancer samples and 6 of 7 (85.7%) cancer cell lines. Aurora-A was overexpressed in 27 of 38 (71.1%) esophageal cancer samples and all 7 esophageal cancer cell lines. We conclude that Aurora-A is amplified and overexpressed in esophageal squamous cancer.
语言:: eng
DOI:

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