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PMID: 17388896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases.

The New phytologist ·Vol. 174 ·No. 2 ·2007-00-00 ·Pages 332-341

Dunand C, Crèvecoeur M, Penel C

Abstract

The respective distribution of superoxide (O(2) (.-)) and hydrogen peroxide (H(2)O(2)), two reactive oxygen species (ROS) involved in root growth and differentiation, was determined within the Arabidopsis root tip. We investigated the effect of changing the levels of these ROS on root development and the possible interactions with peroxidases. H(2)O(2) was detected by confocal laser-scanning microscopy using hydroxyphenyl fluorescein (HPF). Both O(2) (.-) accumulation and peroxidase distribution were assessed by light microscopy, using nitroblue tetrazolium (NBT) and o-dianisidine, respectively. Root length and root hair length and density were also quantified following ROS scavenging. O(2) (.-) was predominantly located in the apoplast of cell elongation zone, whereas H(2)O(2) accumulated in the differentiation zone and the cell wall of root hairs in formation. Treatments that decrease O(2) (.-) concentration reduced root elongation and root hair formation, while scavenging H(2)O(2) promoted root elongation and suppressed root hair formation. The results allow to precise the respective role of O(2) (.-) and H(2)O(2) in root growth and development. The consequences of their distinct accumulation sites within the root tip are discussed, especially in relation to peroxidases.

MeSH Terms
Arabidopsis/enzymology,growth & development,metabolism Hydrogen Peroxide/metabolism Peroxidases/metabolism Plant Roots/enzymology,growth & development,metabolism Seedlings/enzymology,growth & development,metabolism Superoxides/metabolism
Chemicals
Superoxides Hydrogen Peroxide Peroxidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dunand Christophe
Laboratory of Plant Physiology, University of Geneva, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
Crèvecoeur Michèle
Laboratory of Plant Physiology, University of Geneva, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
Penel Claude
Laboratory of Plant Physiology, University of Geneva, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2007.01995.x
Published
2007-00-00
Pages
332-341
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1469-8137.2007.01995.x View journal details
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