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PMID: 17384646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 3' termini of mouse Piwi-interacting RNAs are 2'-O-methylated.

Nature structural & molecular biology ·Vol. 14 ·No. 4 ·2007-04-00 ·Pages 349-50

Ohara T, Sakaguchi Y, Suzuki T, Ueda H, Miyauchi K, Suzuki T

Abstract

Piwi-interacting RNAs (piRNAs) are a germline-specific class of small noncoding RNAs that are essential for spermatogenesis, but their function and biogenesis remain elusive. Here we report a post-transcriptional modification of mouse piRNAs. Mass spectrometric analysis reveals that the piRNAs tested are fully modified by 2'-O-methylation at their 3' termini. This observation may provide a clue to the biogenesis and function of piRNAs in spermatogenesis.

MeSH Terms

Animals Argonaute Proteins Chromatography, Liquid Male Mass Spectrometry Methylation Mice Oxidation-Reduction Proteins/metabolism RNA/chemistry,metabolism RNA Processing, Post-Transcriptional Testis/metabolism

Chemicals

Argonaute Proteins Piwil1 protein, mouse Proteins RNA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Ohara Tomoya

Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.

Sakaguchi Yuriko

Suzuki Takeo

Ueda Hiroki

Miyauchi Kenjyo

Suzuki Tsutomu

Article Info

Journal

Nature structural & molecular biology

Abbr.

Nat Struct Mol Biol

ISSN

1545-9993

DOI

10.1038/nsmb1220

Published

2007-04-00

Epub

2007-00-25

Pages

349-50

Language

English

Region

United States

NLM ID

101186374

Subset

Corrections

CommentIn

PMID: 17410089

External Links

PubMed source DOI: 10.1038/nsmb1220 View journal details

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The 3' termini of mouse Piwi-interacting RNAs are 2'-O-methylated.

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