Home LiteratureArticle Details
PMID: 1734071 Published · ppublish English Journal Article

Evaluation of MicroScan Rapid Pos Combo panels for identification of staphylococci.

Journal of clinical microbiology ·Vol. 30 ·No. 1 ·1992-01-00 ·Pages 93-5

Stoakes L, Schieven BC, Ofori E, Ewan P, Lannigan R, Hussain Z

Abstract

MicroScan Rapid Pos Combo panels (Baxter Diagnostics, Inc., MicroScan, West Sacramento, Calif.) contain substrates conjugated with fluorophores and substrates with a fluorescent pH indicator. AutoSCAn W/A, an automated panel processor equipped with a fluorometer, reads the panels after 2 h of incubation and can identify staphylococci to the species level. We tested 239 strains belonging to 17 species of staphylococci. All the strains were identified by conventional methods (W.E. Kloos and K.H. Schleifer, J. Clin. Microbiol. 1:82-88, 1975) and by the MicroScan Rapid ID system. The system correctly identified 219 (91.6%) strains; nine (3.8%) identification results were probably correct, and six (2.5%) results were incorrect. The system designated five (2.1%) strains as rare biotypes. The automated MicroScan Rapid ID system is useful and reliable in identifying most human isolates of staphylococci encountered in the clinical laboratory.

MeSH Terms
Bacterial Typing Techniques Fluorescent Dyes Humans Microbial Sensitivity Tests Reagent Kits, Diagnostic Staphylococcus/enzymology,isolation & purification Substrate Specificity
Chemicals
Fluorescent Dyes Reagent Kits, Diagnostic
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stoakes L
Department of Clinical Microbiology, Victoria Hospital, London, Ontario, Canada.
Schieven B C
Ofori E
Ewan P
Lannigan R
Hussain Z
References (14)
14 references, click to expand
  1. Comparison of microbiologic characteristics of pathogenic and saprophytic coagulase-negative staphylococci from patients on continuous ambulatory peritoneal dialysis.
    Diagn Microbiol Infect Dis. 1986 Sep;5(3):197-205 PMID: 3638993
  2. Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles.
    J Clin Microbiol. 1981 Mar;13(3):483-90 PMID: 7016897
  3. Comparison of the API Staph-Ident and DMS Staph-Trac systems with conventional methods used for the identification of coagulase-negative staphylococci.
    J Clin Microbiol. 1984 Jan;19(1):68-72 PMID: 6361063
  4. Clinical comparison of the AutoMicrobic system gram-positive identification card, API Staph-Ident, and conventional methods in the identification of coagulase-negative Staphylococcus spp.
    J Clin Microbiol. 1983 Dec;18(6):1323-8 PMID: 6361051
  5. Coagulase-negative staphylococcal bacteremia in patients receiving immunosuppressive therapy.
    Arch Intern Med. 1983 Jan;143(1):32-6 PMID: 6849607
  6. Species identification of coagulase-negative staphylococcal isolates from blood cultures.
    J Clin Microbiol. 1982 Mar;15(3):439-42 PMID: 6918401
  7. A simple test system for the separation of staphylococci from micrococci.
    J Clin Microbiol. 1975 Mar;1(3):337-8 PMID: 1100662
  8. Simplified scheme for routine identification of human Staphylococcus species.
    J Clin Microbiol. 1975 Jan;1(1):82-8 PMID: 170303
  9. Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems.
    J Clin Microbiol. 1991 Apr;29(4):738-44 PMID: 1890175
  10. Methicillin-resistant S. aureus and S. epidermidis strains: modern hospital pathogens.
    Infect Control. 1986 Feb;7(2 Suppl):118-9 PMID: 3633888
  11. Serious postoperative infections caused by coagulase-negative staphylococci: an epidemiological and clinical study.
    J Hosp Infect. 1986 Nov;8(3):233-41 PMID: 2878027
  12. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci.
    Clin Microbiol Rev. 1988 Jul;1(3):281-99 PMID: 3058297
  13. Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci.
    J Clin Microbiol. 1986 Jan;23(1):126-8 PMID: 3517037
  14. Comparison of modified Minitek system with Staph-Ident system for species identification of coagulase-negative staphylococci.
    J Clin Microbiol. 1987 Sep;25(9):1626-8 PMID: 3654937
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1992-01-00
Pages
93-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265002
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com