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PMID: 17335495 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

The use of the zinc-fluorophore, Zinpyr-1, in the study of zinc homeostasis in Arabidopsis roots.

The New phytologist ·Vol. 174 ·No. 1 ·2007-00-00 ·Pages 39-45

Sinclair SA, Sherson SM, Jarvis R, Camakaris J, Cobbett CS

Abstract

* The usefulness of the zinc (Zn)-fluorophore, Zinpyr-1, to examine the localization of Zn in the roots of Arabidopsis has been investigated. * In wild-type roots Zinpyr-1 fluorescence was predominantly in the xylem. The fluorescence signal was abolished by the application of the Zn-chelator, N,N,N',N-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), and was increased by increasing exogenous Zn in the medium, indicating that fluorescence reflected relative Zn concentrations. * In the hma2, hma4 double mutant, which is deficient in root to shoot Zn translocation, Zinpyr-1 fluorescence was low in the xylem and high in the adjacent pericycle cells in which HMA2 and HMA4 are specifically expressed in a wild type. Zinpyr-1 fluorescence was also increased in the endodermis. * These results show that Zinpyr-1 can be used to examine the effects of mutations in Zn transporters on the localization of Zn in Arabidopsis roots and should be a useful addition to the tools available for studying Zn homeostasis in plants.

MeSH Terms
Adenosine Triphosphatases/genetics Arabidopsis/metabolism Arabidopsis Proteins/genetics Carrier Proteins/genetics Chelating Agents/pharmacology Ethylenediamines/pharmacology Feasibility Studies Fluoresceins/metabolism Fluorescent Dyes/metabolism Gene Expression Regulation, Plant Green Fluorescent Proteins/genetics Homeostasis Microscopy, Confocal/methods Plant Roots/metabolism Plants, Genetically Modified Xylem/metabolism Zinc/metabolism
Chemicals
Arabidopsis Proteins Carrier Proteins Chelating Agents Ethylenediamines Fluoresceins Fluorescent Dyes Green Fluorescent Proteins Adenosine Triphosphatases HMA2 protein, Arabidopsis HMA3 protein, Arabidopsis HMA4 protein, Arabidopsis Zinc N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine zinpyr-1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sinclair Scott A
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Sherson Sarah M
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Jarvis Renée
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Camakaris James
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Cobbett Christopher S
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2007.02030.x
Published
2007-00-00
Pages
39-45
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1469-8137.2007.02030.x View journal details
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